این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Microbiology، جلد ۵، شماره ۱، صفحات ۹۱-۹۸
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عنوان انگلیسی A new approach for aggregation of Paramecium caudatum by nitric oxide.
چکیده انگلیسی مقاله Background and Objective: Nitric oxide (NO) plays a role in thermoregulation and growth of protozoa. This work aimed to add the molecule NO in physiology of protozoa in contact with abused narcotic substances. Materials and Methods: A sedative drug, morphine, was infused into a cell chamber containing Paramecia . The cell response to the drug was recorded promptly after drug infusion using a potency protocol provided for the first time at this laboratory. A precursor of NO, L-arginine, was treated jointly with drug to involve the NO system in protozoan performance to drug exposure. Marking of NADPH-diaphorase (NADPH-d) was followed to provide data to explain the mechanisms. Results: Morphine, particularly 0.5 to 60 μg/μl, aggregated the Paramecia . The infusion of L-arginine (1 to 8 µg/µl) together with morphine potentiated this effect, though, pre-usage of L-NAME (1 to 8 µg/µl), a blocker of NO production, reversed the response. Notably the activation of NADPH-d in solely morphine or L-arginine plus morphine samples was revealed. However, the expression of marker was attenuated upon pre-infusion with L-NAME. Conclusion: This study introduces a new approach to involve NO in physiology of aggregation of Paramecia following exposure to the misused sedative drug, morphine.
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نویسندگان مقاله منیژه کرمی | manizheh karami
department of biology, faculty of basic sciences, shahed university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه شاهد (Shahed university)

سید سجاد شاهرخی | seyed sajad shahrokhi
department of biology, faculty of basic sciences, shahed university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه شاهد (Shahed university)

بهرام کاظمی | bahram kazemi
biotechnology amp;amp; molecular biology research center, shahid beheshti university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

سیده سمانه معزی | seyedeh samaneh moezzi
department of biology, faculty of basic sciences, shahed university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه شاهد (Shahed university)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/643
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