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Iranian Journal of Microbiology، جلد ۴، شماره ۳، صفحات ۱۱۸-۱۲۳
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عنوان انگلیسی Molecular identification and detection of virulence genes among Pseudomonas aeruginosa isolated from different infectious origins.
چکیده انگلیسی مقاله Background and Objectives: Pseudomonas aeruginosa possesses a variety of virulence factors that may contribute to its pathogenicity. The aim of this study was to evaluate oprI , oprL and toxA genes for PCR identification of clinical P. aeruginosa . In order to find out any relation between special virulence factors and special manifestation of P. aeruginosa infections, we detected virulence factors among these isolates by PCR. Ribotyping was used to evaluate the clonal relationship between strains with the same genetic patterns of the genes studied. Materials and Methods: In this study, 268 isolates of P. aeruginosa were recovered from burn, wound and pulmonary tract infections. The prevalence of oprI , oprL , toxA , lasB , exoS and nan1 genes was determined by PCR. One hundred and four isolates were selected randomly to investigate clonal diversity of the isolates with ribotyping using Sma I. Results and Conclusions: All P. aeruginosa isolates in this study carried oprI , oprL and lasB genes. Difference between exoS prevalence in isolates from pulmonary tract and burn isolates was statistically significant. Prevalence of nan1 and tox A gene was significantly higher in pulmonary tract and burn isolates, respectively. Ribotyping showed that most of the isolates (87%) belonged to clone A and B.Detection of oprI , oprL and toxA genes by PCR is recommended for molecular identification of P. aeruginosa . Determination of different virulence genes of P. aeruginosa isolates suggests that they are associated with different levels of intrinsic virulence and pathogenicity. Ribotyping showed that strains with the same genetic patterns of the genes do not necessarily have similar ribotype patterns.
کلیدواژه‌های انگلیسی مقاله Pseudomonas aeruginosa,ribotyping,virulence factors
نویسندگان مقاله v s نیک بین | v s nikbin
department of microbiology, pasteur institute of iran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

m m اصلانی | m m aslani
department of microbiology, pasteur institute of iran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

z شرفی | z sharafi
department of microbiology, pasteur institute of iran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

m هاشمی پور | m hashemipour
department of microbiology, pasteur institute of iran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

f شاهچراغی | f shahcheraghi
department of microbiology, pasteur institute of iran, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

g h ابراهیمی پور | g h ebrahimipour
department of biology, shahid beheshti university, iran.

سازمان اصلی تایید شده: دانشگاه شهید بهشتی (Shahid beheshti university)

نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/700
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