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Iranian Journal of Basic Medical Sciences، جلد ۲۰، شماره ۱۱، صفحات ۱۱۹۴-۱۱۹۹
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عنوان انگلیسی Squid ink polysaccharide prevents autophagy and oxidative stress affected by cyclophosphamide in Leydig cells of mice: a pilot study
چکیده انگلیسی مقاله Objective(s): The aim of this study was to explore the effects of Squid ink polysaccharide (SIP) on prevention of autophagy and oxidative stress induced by cyclophosphamide (CP) in Leydig cells of mice. Materials and Methods: Examination of reproductive organ exponents, abnormal sperm rate, activities of superoxide dismutase (SOD), catalase (CAT), contents of malondialdehyde (MDA), and histological structure were performed to detect the optimal dose of SIP against oxidative stress damage in vivo, and autophagy-associated protein LC3 and Beclin-1 were examined by immunofluorescence, and their expression was detected by Western blot analysis. Leydig cells ultrastructural changes were observed by transmission fluorescent microscope. Results: SIP significantly inhibited sperm aberration, histological structure and injury of seminiferous tubules caused by CP, as well as the antioxidant activity of SOD and CAT were increased; contents of MDA were decreased. The optimal dose of SIP for prevention of oxidative stress injury by CP was 80 mg/kg. In addition, LC3 and Beclin-1 fluorescent granules were much less in the Leydig cell layer after treatment via SIP compared with the CP-treated group, and the expression levels of LC3 and Beclin-1 were also decreased. Furthermore, characteristics of cell autophagy such as mitochondrial swelling, autophagic vacuoles, and chromatin pyknosis were observed in CP-treated Leydig cells, but SIP could effectively weaken injury of Leydig cell ultrastructure by CP. Conclusion: SIP, as an antioxidant, prevents the cytoskeleton damage through up-regulation antioxidant capacity and inhibition autophagy caused by CP.
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نویسندگان مقاله yi peng gu | yi peng gu
college of food amp; biological engineering, hezhou university, hezhou 542899, china|college of chemistry amp; environment, guangdong ocean university, zhanjiang 524088, china|school of food science amp; technology, guangdong ocean university, zhanjiang524088, china


محمد بن همام رییس کنفدراسیون mei یانگ | xiao mei yang
college of food amp; biological engineering, hezhou university, hezhou 542899, china


zhen hua duan | zhen hua duan
college of food amp; biological engineering, hezhou university, hezhou 542899, china


jiang hua shang | jiang hua shang
guangxi buffalo research institute, chinese academy of agricultural sciences, nanning 530001, china


ping luo | ping luo
college of chemistry amp; environment, guangdong ocean university, zhanjiang 524088, china


wei محمد بن همام رییس کنفدراسیون | wei xiao
school of food science amp; technology, guangdong ocean university, zhanjiang524088, china


da یان زانگ | da yan zhang
school of food science amp; technology, guangdong ocean university, zhanjiang524088, china


hua zhong liu | hua zhong liu
college of chemistry amp; environment, guangdong ocean university, zhanjiang 524088, china
نشانی اینترنتی http://ijbms.mums.ac.ir/article_9491.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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