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Iranian Journal of Basic Medical Sciences، جلد ۲۰، شماره ۱۰، صفحات ۱۱۳۱-۱۱۴۰
عنوان فارسی
چکیده فارسی مقاله
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عنوان انگلیسی The effect of platelet-rich plasma on human mesenchymal stem cell-induced bone regeneration of canine alveolar defects with calcium phosphate-based scaffolds
چکیده انگلیسی مقاله Objective(s): Autologous bone transplantation known as the "gold standard" to reconstruction of osseous defects has known disadvantages. This study was designed to explore the effects of hydroxy-apatite/tricalcium-phosphate (HA/TCP) and platelet-rich plasma (PRP) on the osteogenesis ability of human adipose-derived mesenchymal stem cells (hAdMSCs) in vitro and in vivo. Materials and Methods: hAdMSCs were incubated with HA/TCP granules and/or PRP in vitro and then, cell proliferation and differentiation was assessed by MTT assay, AZR S staining and SEM examination. In vivo, four cylindrical defects were drilled in the mandibular bones of 5 mongrel dogs and divided randomly into the following groups: I-autologous crushed bone, II- no filling material, III- HA/TCP and PRP, IV- PRP-enriched hAdMSCs seeded on HA/TCP granules. Inserted hAdMSCs were labeled to trace their contribution to bone tissue regeneration. Finally, cell tracing and tissue regeneration were evaluated by immunohistochemistry and histomorphometry methods, respectively.    Results: In vitro, co-incubation with HA/TCP granules significantly reduced proliferation and osteogenic differentiation ability of hAdMSCs; while PRP application promoted these capacities (P<0.05). In vivo, PRP-enriched hAdMSCs seeded on HA/TCP granules induced considerable bone formation in osseous defects (P<0.05). It was obviously shown that hAdMSCs were incorporated into the newly-formed bone.    Conclusion: Based on this study, application of stem cells could offer a helpful therapeutic tool in bone tissue regeneration. Although inserted hAdMSCs were identifiable throughout the newly-formed bone tissue, their few number could be an indicator of indirect role of hAdMSCs in tissue regeneration.
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نویسندگان مقاله ریحانه شفیعیان | reihaneh shafieian
department of anatomy and cell biology, school of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

مریم مقدم متین | maryam moghaddam matin
stem cell and regenerative medicine research group, academic center for education, culture, and research acecr , khorasan razavi branch, mashhad, iran


امین راه پیما | amin rahpeyma
oral and maxillofacial diseases research center, school of dentistry, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

علیرضا فاضل | alireza fazel
microanatomy research center, school of medicine, mashhad university of medical sciences, mashhad, iran|department of anatomy and cell biology, school of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

حمیده سالاری صدیق | hamideh salari sedigh
department of clinical sciences, faculty of veterinary medicine, ferdowsi university of mashhad, mashhad, iran

سازمان اصلی تایید شده: دانشگاه فردوسی (Ferdowsi university)

آریانه صدر نبوی | ariane sadr nabavi
department of genetics, school of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

حلیمه حسن زاده | halimeh hassanzadeh
stem cell and regenerative medicine research group, academic center for education, culture, and research acecr , khorasan razavi branch, mashhad, iran


علیرضا ابراهیم زاده بیدسکان | alireza ebrahimzadeh bideskan
microanatomy research center, school of medicine, mashhad university of medical sciences, mashhad, iran|department of anatomy and cell biology, school of medicine, mashhad university of medical sciences, mashhad, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_9447.html
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