| چکیده انگلیسی مقاله |
Objective(s):Rapamycin is an immunosuppressant compound with a broad spectrum of pharmaco-logical activities. In recent years, it has been used successfully to decrease ischemia-reperfusion injury in several organ systems. The purpose of the present study was to examine the effect of rapamycin on testicular ischemia-reperfusion injury. Materials and Methods: Seventy-two adult male Wistar rats were divided into six groups: control (group1), sham-operated (Group2), T/D + DMSO as vehicle group (group3), and groups 4–6; respectively received 0.5, 1, and 1.5 mgkg-1 of rapamycin , IP 30 min before detorsion. Ischemia was achieved by twisting the right testis 720o clockwise for 1 hr. The right testis of 6 animals from each group were excised 4 hr after detorsion for the measurement of lipid peroxidation, caspase-3, and antioxidant enzyme activities. Histopathological changes and germ cell apoptosis were determined by measuring mean of seminiferous tubules diameters (MSTD) and TUNEL test in right testis of 6 animals per group, 24 hr after detorsion. Results: Testicular T/D caused increases in the apoptosis, malondialdehyde (MDA), and caspase-3 levels and decreases in the superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activities in ipsilateral testis (P< 0.001). The rats treated with rapamycin had significant decreases in the MDA and caspase-3 levels and significant increases in the SOD, CAT and GPx activities in ipsilateral testis compared with the T/D group (P< 0.001); germ cell apoptosis was decreased, and MSTD was improved. Conclusion: Rapamycin administration during testicular torsion decreased ischemia/reperfusion (I/R) cellular damage. |
| نویسندگان مقاله |
مرتضی قاسم نژادبرنجی | morteza ghasemnejad berenji
department of pharmacology, school of medicine, tehran university of medical sciences|experimental medicine research center, tehran university of medical sciences|department of pharmacology, faculty of pharmacy, urmia university of medical sciences
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
محمود قاضی خوانساری | mahmoud ghazi khansari
department of pharmacology, school of medicine, tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
ایرج یزدانی | iraj yazdani
department of pharmacology, school of medicine, tehran university of medical sciences, tehran, iran|experimental medicine research center, tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
سید سهیل سعیدی ساروی | seyed soheil saeedi saravi
department of pharmacology, school of medicine, tehran university of medical sciences, tehran, iran|experimental medicine research center, tehran university of medical sciences, tehran, iran|department of pharmacology, faculty of pharmacy, guilan university of medical sciences, rasht, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی گیلان (Guilan university of medical sciences)
ملیحه نوبخت | maliheh nobakht
department of anatomy, school of medicine, iran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
علیرضا عبداللهی | alireza abdollahi
department of pathology, cancer institute, tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
جواد مهاجر انصاری | javad mohajer ansari
department of anatomy, school of medicine, iran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)
حجت قاسم نژادبرنجی | hojjat ghasemnejad berenji
department of anatomy and reproductive biology, school of medicine, shahid beheshti university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)
سروین پاشاپور | sarvin pashapour
department of pathology, urmia university of medical sciences, urmia, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی ارومیه (Urmia university of medical sciences)
احمدرضا دهپور | ahmad reza dehpour
department of pharmacology, school of medicine, tehran university of medical sciences, tehran, iran|experimental medicine research center, tehran university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
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