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Iranian Journal of Basic Medical Sciences، جلد ۲۰، شماره ۸، صفحات ۹۱۲-۹۱۹
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عنوان انگلیسی Detection of tetracycline resistance genes, aminoglycoside modifying enzymes, and coagulase gene typing of clinical isolates of Staphylococcus aureus in the Southwest of Iran
چکیده انگلیسی مقاله Objective(s): The aim of the present study was to determine the aminoglycoside modifying enzymes (AMEs) encoded genes, tetracycline resistance genes, and the coa based typing of Staphylococcus aureus isolates in the Southwest of Iran. Materials and Methods: Antimicrobial susceptibility of isolates was carried out by agar disk diffusion methods. Two sets of multiplex PCR mixture were used for detection of AME genes and tet genes.  All of the isolates were typed with the coagulase gene typing method. Of the 121 isolates, 29.75% and 47.93% were resistant to at least one aminoglycosides and tetracyclines, respectively. Results: The aac(6')-Ie-aph(2'') was the most frequent gene (97.22%), and aph (3')-IIIa and ant (4')-Ia genes were detected in 61.11% and 11.11% of aminoglycoside resistant isolates, respectively. The tetK and tetM genes were detected in 82.75% and 56.9% of tetracycline resistant isolates, respectively. Overall 31.4% of isolates were MRSA. Totally 17 distinct coa gene RFLP patterns, numbered C1 to C17, were observed.  The C5 was the most frequent coa type with 31 isolates. Conclusion: The aac(6')-Ie-aph(2'') and aph (3')-IIIa genes were the most important genes contributing to aminoglycosides resistance, while resistance to tetracyclines was mediated by tetK and tetM genes. Interestingly all S. aureus with C5 as the most prevalent coa-type were resistant to at least one of the aminoglycoside antibiotics and tetracycline simultaneously. Moreover, 30 out of 31 isolates with this coa type were MRSA, indicating the importance of the C5 coa-type in MRSA strains and also in isolates that were resistant to aminoglycosides and tetracycline.
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نویسندگان مقاله سید سجاد خرم روز | seyed sajjad khoramrooz
cellular and molecular research center, yasuj university of medical sciences, yasuj, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی یاسوج (Yasouj university of medical sciences)

ثمین علیپور dolatabad | samin alipoor dolatabad
department of biology, faculty of science, karaj branch, islamic azad university, karaj, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

فاطمه مصطفی پور dolatabad | fatemeh mostafapour dolatabad
department of basic sciences, islamic azad university, yasooj branch, yasooj, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

مسعود مرعشی فرد | masoud marashifard
student research committee, yasuj university of medical sciences, yasuj, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی یاسوج (Yasouj university of medical sciences)

مهدی میرزایی | mehdi mirzaii
school of medicine, shahroud university of medical sciences, shahroud, iran


حسین دبیری | hossein dabiri
department of medical microbiology, shahid beheshti university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی شهید بهشتی (Shahid beheshti university of medical sciences)

اعظم حدادی | azam haddadi
department of biology, faculty of science, karaj branch, islamic azad university, karaj, iran

سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

سید محمدرضا ربانی | seyed mohammadreza rabani
beheshti teaching hospital, yasuj university of medical sciences, yasuj, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی یاسوج (Yasouj university of medical sciences)

حمیدرضا غفاریان شیرازی | hamid reza ghaffarian shirazi
cellular and molecular research center, yasuj university of medical sciences, yasuj, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی یاسوج (Yasouj university of medical sciences)

داود دربان ساروخلیل | davood darban sarokhalil
department of microbiology, school of medicine, iran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_9114.html
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