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Iranian Journal of Basic Medical Sciences، جلد ۲۰، شماره ۷، صفحات ۷۹۱-۷۹۷
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Heterologous expression of a truncated form of human recombinant vascular endothelial growth factor-A and its biological activity in wound healing
چکیده انگلیسی مقاله Objective(s): Vascular endothelial growth factor (VEGF) is one of the most effective proteins in angiogenesis, mesenchymal stem cells (MSCs) differentiation and wound healing. These abilities are therapeutic potential of VEGF in diabetic retinopathy, nephropathy and other tissue damage circumstances. In this study, recombinant VEGF was produced in Escherichia coli (E. coli) system and then biological activity of this protein was evaluated in animal wound healing. Materials and Methods: E. coli BL21 (DE3) competent cells were transformed with pET32a-VEGF clone and induced by isopropyl-β-D-thio-galactoside (IPTG). The recombinant protein was purified byaffinity chromatography. Recombinant VEGF-A-based ointment (VEGF/Vaseline 0.8 mg/100 w/w) was used for external wound (25×15mm thickness) healing in animal model. In vivo activity of ointment was evaluated by clinical evidences and cytological microscopic assessment. Results: The recombinant protein with molecular weight of 45 kilodaltons (kDa) and concentration of 0.8 mg/ml was produced.Immunoblotting data showed that the antigenic region of VEGF can be expressed in E. coli and the recombinant protein has similar epitopes with close antigenic properties to the natural form. Macroscopic findings and microscopic data showed that the recombinant VEGF-A ointment was effective on excisional wound healing. Conclusion: Recombinant VEGF-A produced by pET32a in E. coli, possesses acceptable structure and has wound healing capability.
کلیدواژه‌های انگلیسی مقاله Biological activity, Recombinant, VEGF-A, Wound healing
نویسندگان مقاله محسن خاکی | mohsen khaki
molecular and medicine research center, department of immunology and microbiology, school of medicine, arak university of medical sciences, arak, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اراک (Arak university of medical sciences)

علی هاتف سلمانیان | ali hatef salmanian
national institute for genetic engineering and biotechnology, tehran, iran


قاسم مسیبی | ghasem mosayebi
molecular and medicine research center, department of immunology and microbiology, school of medicine, arak university of medical sciences, arak, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اراک (Arak university of medical sciences)

مریم باعزم | maryam baazm
department of anatomy, school of medicine, arak university of medical sciences, arak. iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اراک (Arak university of medical sciences)

سعید بابایی | saeed babaei
department of anatomy, school of medicine, arak university of medical sciences, arak. iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اراک (Arak university of medical sciences)

ندا مولایی | neda molaee
molecular and medicine research center, department of immunology and microbiology, school of medicine, arak university of medical sciences, arak, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اراک (Arak university of medical sciences)

حمید ابطحی | hamid abtahi
molecular and medicine research center, department of immunology and microbiology, school of medicine, arak university of medical sciences, arak, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اراک (Arak university of medical sciences)

نشانی اینترنتی http://ijbms.mums.ac.ir/article_9011.html
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زبان مقاله منتشر شده en
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