این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Basic Medical Sciences، جلد ۲۰، شماره ۴، صفحات ۴۰۸-۴۱۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Naringin enhances osteogenic differentiation through the activation of ERK signaling in human bone marrow mesenchymal stem cells
چکیده انگلیسی مقاله Objective(s): Naringin has been reported to regulate bone metabolism. However, its effect on osteogenesis remains unclear. The aim was to investigate the effect of naringin on osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) through the activation of the ERK signaling pathway in osteogenic differentiation. Materials and Methods: Annexin V-FITC assay and MTT assay were used to measure the effect of naringin on cytotoxicity and proliferation of hBMSCs, respectively. Alkaline phosphatase activity analysis, Alizarin Red S staining, Western blotting, and real-time PCR assay were used to evaluate both the potential effect of naringin on osteogenic differentiation and the role of ERK signaling pathway in osteogenic differentiation. Results: Our results showed that naringin had no obvious toxicity on hBMSCs, and could significantly promote the proliferation of hBMSCs. Naringin also enhanced the osteogenic differentiation of hBMSCs and increased the protein and mRNA expression levels of osteogenic markers such as Runx-2, OXS, OCN, and Col1 in a dose-dependent manner. In addition, we found that the enhancing effect of naringin on osteogenic differentiation was related to the activation of phosphor-ERK, with an increase in duration of activity from 30 min to 120 min. More importantly, both the enhancing effect of naringin on osteogenic differentiation and the activity effect of naringin on ERK signaling pathway were reversed by U0126 addition. Conclusion: Our findings demonstrated that naringin promoted proliferation and osteogenesis of hBMSCs by activating the ERK signaling pathway and it might be a potential therapeutic agent for treating or preventing osteoporosis.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله huichao ونگ | huichao wang
luoyang orthopedic hospital of henan province, orthopedic institute of henan province, luoyang 471002, china


chunbo li | chunbo li
shanghai first maternity and infant hospital, tongji university of medicine. shanghai, 200126, china


jianming li | jianming li
luoyang orthopedic hospital of henan province, orthopedic institute of henan province, luoyang 471002, china


yingjie zhu | yingjie zhu
luoyang orthopedic hospital of henan province, orthopedic institute of henan province, luoyang 471002, china


yudong جیا | yudong jia
luoyang orthopedic hospital of henan province, orthopedic institute of henan province, luoyang 471002, china


ying زانگ | ying zhang
luoyang orthopedic hospital of henan province, orthopedic institute of henan province, luoyang 471002, china


xiaodong زانگ | xiaodong zhang
luoyang orthopedic hospital of henan province, orthopedic institute of henan province, luoyang 471002, china


wenlong li | wenlong li
henan university of traditional chinese medicine, henan province, zhengzhou 450000, china


lei cui | lei cui
medical science amp; research center, beijing shijitan hospital affiliated to capital medical university, 10 tieyi road, beijing 100038, china


wuyin li | wuyin li
luoyang orthopedic hospital of henan province, orthopedic institute of henan province, luoyang 471002, china


youwen liu | youwen liu
luoyang orthopedic hospital of henan province, orthopedic institute of henan province, luoyang 471002, china
نشانی اینترنتی http://ijbms.mums.ac.ir/article_8582.html
فایل مقاله دریافت فایل مقاله
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات