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Avicenna Journal of Phytomedicine، جلد ۷، شماره ۶، صفحات ۵۱۹-۵۳۰
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عنوان انگلیسی In vitro and in vivo effects of Peganum harmala L. seeds extract against influenza A virus
چکیده انگلیسی مقاله Objective: Influenza A virus infections are still a major health problem and the choices available for the control and treatment of the disease are limited. This research evaluated in vitro and in vivo antiviral effects of Peganum harmala L. seeds (PHS) extract against influenza A virus. Materials and Methods: In this research, in vitro anti-influenza A virus activity of the extract was assessed in Madin-Darby canine kidney (MDCK) cells. In order to evaluate anti-influenza activity of PHS extract in vivo, BALB/c mice were infected with 5LD50 of mouse-adapted influenza virus (H1N1; PR8) and received 200 mg/kg/day of PHS extract or 20 mg/kg/day oseltamivir. Lungs of seven mice per group were removed on day 3 post-infection and lung virus titers were determined by qRT-PCR. Mice survival, body weights and general conditions were observed for up to 14 days post-infection. Results: The results demonstrated that, the ethanolic extract of PHS possesses high activity against influenza virus with IC50 value of 15.7 (CI95%:11.7-21) μg/ml in MDCK cells. Our results also showed that, oral administration of PHS extract (200 mg/kg/day) or oseltamivir (20 mg/kg/day) to infected mice, increased the survival rate, reduced body weight loss, and decreased lung virus titer. Conclusion: Based on our findings, P. harmala seeds extract can inhibit influenza A virus replication in vitro and in vivo. Therefore, isolation and characterization of the plant's active compounds and investigation of the underlying mechanisms of its antiviral action are highly suggested.
کلیدواژه‌های انگلیسی مقاله Influenza A virus, Antiviral activity, Peganum harmala L
نویسندگان مقاله محمد تقی مرادی | mohammad taghi moradi
students research committee, medical plants research center, shahrekord university of medical science, shahrekord, iran


علی کریمی | ali karimi
medical plants research center, basic health sciences institute, shahrekord university of medical sciences, shahrekord, iran


فاطمه فتوحی | fatemeh fotouhi
department of influenza and other respiratory viruses, pasteur institute of iran, tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

سلیمان خیری | soleiman kheiri
department of epidemiology and biostatistics, shahrekord university of medical sciences, shahrekord, iran


علی ترابی | ali torabi
department of influenza and other respiratory viruses, pasteur institute of iran, tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

نشانی اینترنتی http://ajp.mums.ac.ir/article_9060.html
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Research Article
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