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Iranian Journal of Biotechnology، جلد ۱۵، شماره ۱، صفحات ۶۷-۷۳
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عنوان انگلیسی Partial Purification and Characterization of the Recombinant Benzaldehyde Dehydrogenase from Rhodococcus ruber UKMP-5M
چکیده انگلیسی مقاله Background: Benzaldehyde dehydrogenase (BZDH) is encoded by the xylC that catalyzes the conversion of benzaldehyde into benzoate in many pathways such as toluene degradation. Objectives: In this study, the xylC gene from Rhodococcus ruber UKMP-5M was expressed in Escherichia coli, purified, and characterized.Materials and Methods: The xylC was amplified and cloned in E. coli. The recombinant plasmid pGEMT-xylC was digested by NdeI and HindIII to construct plasmid pET28b-xylC and transformed in E. coli BL21 (DE3). Expression of the recombinant protein was induced by 1 mM isopropyl β-D-thiogalactoside (IPTG) at 37 °C. The BZDH was purified by ion exchange chromatography, in which the product was an NAD-dependent enzyme using benzaldehyde as a substrate for enzyme characterization. The end metabolite was identified via gas chromatography mass spectrometry (GC-MS). Results: The recombinant BZDH is 27 kDa, purified by ion exchange chromatography. The activity of BZDH was 9.4 U/ml. The optimum pH and temperature were 8.5 and 25 ºC, respectively. The Michaelis constant (Km) and maximum velocity (Vmax) were 4.2 mM and 19.7 U/ml, respectively. The metabolite of BZDH was benzene carboxylic acid as determined by GC-MS analysis. Conclusion: BZDH has the ability to degrade benzaldehyde to less toxic compounds. The BZDH is a critical enzyme for the degradation of aromatic hydrocarbons in Rhodococcus sp. The BZDH from R. ruber UKMP-5M is showed similar function with other aldehyde dehydrogenases.
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نویسندگان مقاله آرزو توکلی |
assistant professor in islamic azad university eghlid branch, eghlid, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)

ainon حمزه |
school of biosciences and biotechnology, faculty science and technology, national university of malaysia, selangor, malaysia

نشانی اینترنتی http://www.ijbiotech.com/article_44225_cea4125ec4706e798960978359ea3a81.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/441/article-441-485019.pdf
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