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Research in Pharmaceutical Sciences، جلد ۱۲، شماره ۶، صفحات ۴۵۶-۴۶۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی The silencing effect of miR-30a on ITGA4 gene expression in vitro: an approach for gene therapy
چکیده انگلیسی مقاله Integrins are adhesion molecules which play crucial roles in cell-cell and cell-extracellular matrix interactions. Very late antigen-4 or α4β1 and lymphocyte Peyer’s patch adhesion molecule-1 or α4β7, are key factors in the invasion of tumor cells and metastasis. Based on the previous reports, integrin α4 ( ITGA4 ) is overexpressed in some immune disorders and cancers. Thus, inhibition of ITGA4 could be a therapeutic strategy. In the present study, miR-30a was selected in order to suppress ITGA4 expression. The ITGA4 3´UTR was amplified, cloned in the Z2827-M67-( ITGA4 ) plasmid and named as Z2827-M67/3´UTR. HeLa cells were divided into five groups; (1) untreated without any transfection, (2) mock with Z2827-M67/3´UTR transfection and X-tremeGENE reagent, (3) negative control with Z2827-M67/3´UTR transfection alone, (4) test with miR-30a mimic and Z2827-M67/3´UTR transfection and (5) scramble with miR-30a scramble and Z2827-M67/3´UTR transfection. The MTT assay was performed to evaluate cell survival and cytotoxicity in each group. Real-time RT-PCR was applied for the ITGA4 expression analysis. The findings of this study showed that miR-30a downregulated ITGA4 expression and had no effect on the cell survival. Due to the silencing effect of miR-30a on the ITGA4 gene expression, this agent could be considered as a potential tool for cancer and immune disorders therapy.
کلیدواژه‌های انگلیسی مقاله Cancer,ITGA4,Metastasis,miR-30a, Non-coding RNA
نویسندگان مقاله لیلا درزی | leila darzi
2isfahan cardiovascular research center, cardiovascular research institute, isfahan university of medical sciences, isfahan, i.r. iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

مریم بشتام | maryam boshtam
2isfahan cardiovascular research center, cardiovascular research institute, isfahan university of medical sciences, isfahan, i.r. iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

لاله شریعتی | laleh shariati
3department of immunology, school of medicine, isfahan university of medical sciences, isfahan, i.r. iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

شیرین کوهپایه | shirin kouhpayeh
4department of medical biotechnology, school of advanced technologies in medicine, tehran university of medical sciences, tehran, i.r. iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

اعظم غیبی | azam gheibi
5department of pharmaceutical biotechnology and isfahan pharmaceutical science research center, school of pharmacy and pharmaceutical science, isfahan university of medical sciences, isfahan, i.r. iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

مینا میریان | mina mirian
1department of genetics and molecular biology, school of medicine, isfahan university of medical sciences, isfahan, i.r. iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

ایلناز رحیم منش | ilnaz rahimmanesh
1department of genetics and molecular biology, school of medicine, isfahan university of medical sciences, isfahan, i.r. iran. 6pediatric inherited diseases research center, research institute for primordial prevention of noncommunicable disease, isfahan university of medical sciences, isfahan, i.r. iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

حسین خان احمد | hossein khanahmad
1department of genetics and molecular biology, school of medicine, isfahan university of medical sciences, isfahan, i.r. iran. 6pediatric inherited diseases research center, research institute for primordial prevention of noncommunicable disease, isfahan university of medical sciences, isfahan, i.r. iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی اصفهان (Isfahan university of medical sciences)

محمد امین طباطبایی فر | mohammad amin tabatabaiefar


نشانی اینترنتی http://rps.mui.ac.ir/index.php/jrps/article/view/1792
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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