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Iranian Journal of Blood and Cancer، جلد ۷، شماره ۲، صفحات ۱۱۱-۱۱۶
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عنوان انگلیسی A New Two Step Induction Protocol for Neural Differentiation of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells
چکیده انگلیسی مقاله Background: In this study, we examined a new two step induction protocol for improving the differentiation of human umbilical cord blood-derived mesenchymal stem cells into neural progenitor cells. Materials and Methods: Human umbilical cord blood-derived mesenchymal stem cells were first cultured in Dulbecco’s modified eagle medium supplemented with 10% fetal bovine serum in a humidified incubator equilibration with 5% CO2 at 37oC. To stimulate neural-differentiation of human umbilical cord blood-derived mesenchymal stem cells, Dulbecco’s modified eagle medium was removed and replaced with pre-induction media including retinoic acid, basic fibroblast growth factor, epidermal growth factor, and basal medium for 2 days. Then, we used nerve growth factor, 3-isobutylmethyl-xanthine, ascorbic acid, and basal medium for 6 days. Real-time PCR was performed to analyze the expression of neural specific genes such as glial fibrillary acidic protein, microtubule-associated protein 2, major basic protein, and Nestin. Results: Real-time-PCR showed that the expression of glial fibrillary acidic protein, major basic protein, and microtubule-associated protein 2 genes, after two step induction, significantly increased compared to common induction protocol. In addition, our study showed that retinoic acid might play a main role in neural-differentiation and fate of mesenchymal stem cells compared to other neural inducers. Conclusion: The combination of chemicals and growth factors in a two step induction protocol may improve the efficiency of differentiation of human umbilical cord blood-derived mesenchymal stem cells into neural progenitor cells and provide a new method for easy and fast application of human umbilical cord blood-derived mesenchymal stem cells in regenerative medicine. Keywords: Umbilical cord blood, mesenchymal stem cell, neural progenitor cells, neural-differentiation.
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نویسندگان مقاله رفیعی مهر h | rafieemehr h
blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran.

سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)

خیراندیش m | kheirandish m
blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran.

سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)

سلیمانی m | soleimani m
faculty of medicine, tarbiat modares university, tehran, iran.

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)
سازمان های دیگر: Stem Cell Technology Research Center, Tehran, Iran.

نشانی اینترنتی http://www.ijbc.ir/browse.php?a_code=A-10-9-45&slc_lang=en&sid=en
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