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Iranian Journal of Blood and Cancer، جلد ۶، شماره ۴، صفحات ۱۶۹-۱۷۵
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عنوان انگلیسی Microparticle Formation and Platelet Shrinkage in Type-I Glanzmman Thrombasthenia Platelets
چکیده انگلیسی مقاله Background: Activated normal platelets undergo many biochemical and morphological changes, some of which are apoptotic. Platelet derived microparticles and shrinked platelets as hallmark of platelet activation and apoptosis disperse surfaces containing procoagulant activity around injured vessels and tissues. This study was conducted to determine microparticles formation and platelet shrinkage in Glanzmann thrombasthenia upon activation. Patients and Methods: Platelets from twelve unrelated type I Glanzmann thrombasthenia patients were examined as washed platelets. Calcium ionophore A23187 was used as agonist to activate the platelets. Flow cytometry was applied to measure platelet-derived micro particles (forward scatter events < 1.0 µm size), and platelet shrinkage (mean-FSC). Anti-CD42b was used as platelet specific marker to distinguish platelets from other likely particles. Annexin A5 Alexa Fluor was used to determine phosphatidylserine exposure and confirm platelet activation and apoptosis. Results: Calcium ionophore A23187, dramatically increased MP formation by type-I GT platelets up to 14.5 fold increase over baseline (Buffer treated: 14.18 ± 5.4% vs. A23187 treated: 34.31 ± 15.2% p< 0.005). Also calcium ionophore A23187, increased platelet shrinkage by type-I GT platelets and mean-FSC decreased (Buffer treated: 4.12±1.3 vs. A23187 treated: 1.67±0.2 p< 0.0024). Conclusion: This study showed that, type I Glanzmann thrombasthenia platelets demonstrate platelet apoptosis considering two apoptotic targets, including micro particles formation and platelet shrinkage. We conclude that in thrombasthenic Glanzmann platelets at least some aspects of normal apoptosis is ongoing, and this may explain normal platelet count among these patients. Keywords: Apoptosis, Glanzmann thrombasthenia, glycoprotein IIbIIIa, flow cytometry, microparticle.
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نویسندگان مقاله همتی t | hemmati t
research institute of transfusion medicine. blood transfusion research center, high institute for research and education

سازمان اصلی تایید شده: سازمان انتقال خون ایران (Blood transfusion research center)

فارسی نژاد a | farsinezhad a
school of allied health sciences, kerman university of medical sciences, kerman, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی کرمان (Kerman university of medical sciences)

محمد رضا mr | managhchi mr
imam khomeini hospital, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

توگه g | toogeh g
imam khomeini hospital, tehran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

فرانوش m | faranoush m
iran university of medical sciences, tehran, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

نشانی اینترنتی http://www.ijbc.ir/browse.php?a_code=A-10-1-100&slc_lang=en&sid=en
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