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Acta Medica Iranica، جلد ۵۵، شماره ۹، صفحات ۵۳۹-۵۴۹
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عنوان انگلیسی MMP9 Gene Expression Variation by Ingesting Tart Cherry and P-Coumaric Acid During Remyelination in the Cuprizone Mouse Model
چکیده انگلیسی مقاله Matrix metalloproteinase-9 (GELB) as a member of gelatinases plays key role in the destruction of blood-brain barrier (BBB), T cells migration into the CNS, and demyelination induction. Considering remyelination induction in response to tart cherry extract and pure p-coumaric acid ingestion via tracking MMP9 gene expression in the cuprizone mouse model. Firstly, predicting the chemical interaction between p-coumaric acid and MMP9 protein was conducted through PASS and Swiss dock web services. Next, the content of p-coumaric acid in the tart cherry extract was analyzed by HPLC. Later, mice (male, female) were categorized into two groups: standard, cuprizone. After the demyelination period, mice classified into four groups: standard, natural chow, tart cherry extract, p-coumaric acid. Finally, brains were extracted from the skull, and MMP9 gene expression was evaluated by real time RT-PCR. Bioinformatics analysis displayed p-coumaric acid has potent inhibitory effect on MMP9 gene expression (Pa=0.818) with estimated ΔG (kcal/mol) -8.10. In addition, during the demyelination period, MMP9 expression was increased significantly in the male group that is related to myelin destruction. However, MMP9 was declined throughout remyelination in both male and female. It’s remarkable that pure p-coumaric acid and tart cherry extract ingestion could decrease the gene expression ratio more than natural chow. According to the results, it’s deduced the male mouse is more appropriate gender for demyelination induction via cuprizone. In addition, tart cherry extract and pure p-coumaric acid ingestion could decrease MMP9 gene expression level considerably during remyelination.
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نویسندگان مقاله newshan بهرنگی | newshan behrangi
pasteur institute, production and research complex, karaj, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

نبی اله نامور | nabiyollah namvar
national institute of genetic engineering and biotechnology, tehran, iran.

سازمان اصلی تایید شده: پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری

میترا عطایی | mitra ataei
pasteur institute, production and research complex, karaj, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

سکینه دیزجی | sakineh dizaji
pasteur institute, production and research complex, karaj, iran.

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

گلشید جاودانی | golshid javdani
national institute of genetic engineering and biotechnology, tehran, iran.

سازمان اصلی تایید شده: پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری

محمد حسین صنعتی | mahommad hossein sanati


نشانی اینترنتی http://acta.tums.ac.ir/index.php/acta/article/view/5896
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