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Journal of Arthropod-Borne Diseases، جلد ۱۱، شماره ۴، صفحات ۵۱۵-۵۳۲
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عنوان انگلیسی Dynamics of Transgenic Enterobacter cloacae Expressing Green Fluorescent Protein-Defensin (GFP-D) in Anopheles stephensi under Laboratory Condition
چکیده انگلیسی مقاله Background: Enterobacter cloacae bacterium is a known symbiont of the most Anopheles gut microflora and nominated as a good candidate for paratransgenic control of malaria. However, the population dynamics of this bacterium with­in An. stephensi and its introduction methods to the mosquitoes have not yet been explored. Methods: Enterobacter cloacae subsp. dissolvens expressing green fluorescent protein and defensin (GFP-D) was used to study transstadial transmission and the course of time, larval habitat, sugar, and blood meal on dynamics of the bacterium in the mosquito life stages in the laboratory condition. The bacterial quantities were measured by plating samples and counting GFP expressing colonies on the Tet-BHI agar medium. Results: The E. cloacae population remained stable in sugar bait at least for eleven days whereas it was lowered in the insectary larval habitat where the bacteria inadequately recycled. The bacterium was weakly transmitted transstadi­ally from larval to adult stage. The bacterial populations increased smoothly and then dramatically in the guts of An. stephensi following sugar and blood meal respectively followed by a gradual reduction over the time. Conclusion: Enterobacter cloacae was highly stable in sugar bait and increased tremendously in the gut of female adult An. stephensi within 24h post blood meal. Sugar bait stations can be used for introduction of the transgenic bacteria in a paratransgenic approach. It is recommended to evaluate the attraction of sugar bait in combination with attractive kairomones as well as its stability and survival rate in the semi-field or field conditions.
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نویسندگان مقاله حسین دهقان | hossein dehghan
department of medical entomology and vector control, school of public health, tehran university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمد علی عشاقی | mohammad ali oshaghi
department of medical entomology and vector control, school of public health, tehran university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

سید حسن موسی کاظمی | seyed hassan moosa kazemi
department industrial and of environmental biotechnology, national institute of genetic engineering and biotechnology, tehran, iran

سازمان اصلی تایید شده: پژوهشگاه ملی مهندسی ژنتیک و زیست فناوری

باقر یخچالی | bagher yakhchali
department of medical entomology and vector control, school of public health, tehran university of medical sciences, iran department of chemical pollutants and pesticides, institute for environmental research, tehran university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

حسن وطن دوست | hassan vatandoost
malaria and vector research group, biotechnology research center, pasteur institute of iran, tehran, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

ناصح ملکی rlm رواسان | naseh maleki ravasan
department of medical entomology and vector control, school of public health, tehran university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

یاور راثی | yavar rassi
department of parasitology and mycology, faculty of medicine, urmia university of medical sciences, urmia, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ارومیه (Urmia university of medical sciences)

حبیب محمدزاده | habib mohammadzadeh
department of medical entomology and vector control, school of public health, tehran university of medical sciences, iran department of chemical pollutants and pesticides, institute for environmental research, tehran university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

محمدرضا عبایی | mohammad reza abai
department of medical entomology and vector control, school of public health, tehran university of medical sciences, iran

سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)

فاطمه محترمی | fatemeh mohtarami


نشانی اینترنتی http://jad.tums.ac.ir/index.php/jad/article/view/778
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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