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Nanomedicine Journal، جلد ۵، شماره ۱، صفحات ۴۶-۵۱

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عنوان انگلیسی Comparative characterization of silver nanoparticles synthesized by spore extract of Bacillus subtilis and Geobacillus stearothermophilus
چکیده انگلیسی مقاله Objective(s): Silver nanostructures have gathered remarkable attention due to their applications in diversefields. Researchers have recently demonstrated that bacterial spores are capable of reducing silver ions toelemental silver leading to formation of nanoparticles.Materials and Methods: In this study, spores of Bacillus subtilis and Geobacillus stearothermophilus wereemployed to produce silver nanoparticles (SNPs) from silver nitrate (AgNO3) through a green synthesismethod. The production of SNPs by spores, heat inactivated spores (microcapsule) and spore extracts wasmonitored and compared at wavelengths between 300 to 700 nm. The biosynthesized SNPs by spore extractswere characterized and confirmed by XRD and TEM analyses.Results: UV-Visible spectroscopy showed that the spore extracts were able to synthesize more SNPs thanthe other forms. The XRD pattern also revealed that the silver nanometals have crystalline structure withvarious topologies. The TEM micrographs showed polydispersed nanocrystal with dimensions ranging from30 to 90 nm and 15 to 50 nm produced by spore extracts of B. subtilis and G. stearothermophilus, respectively.Moreover, these biologically synthesized nanoparticles exhibited antimicrobial activity against differentopportunistic pathogens.Conclusion: This study suggests the bacterial spore extract as a safe, efficient, cost effective and eco-friendlymaterial for biosynthesis of SNPs.
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نویسندگان مقاله سید مهدی قاسمی | seyed mahdi ghasemi
department of medical mycology, aja university of medical sciences, tehran, iran| department of biotechnology, faculty of biological sciences and technology, shahid ashrafi esfahani university, isfahan, iran


بنفشه درمنش | banafshe dormanesh
department of pediatric nephrology, aja university of medical sciences, tehran, iran


afrouzossadat حسینی ابری | afrouzossadat hosseini abari
department of biology, faculty of science, university of isfahan, isfahan, iran

سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

اعظم علی اصغری | azam aliasghari
department of biology, faculty of science, university of isfahan, isfahan, iran.

سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

زهره فرح نژاد | zohreh farahnejad
department of medical mycology, aja university of medical sciences, tehran,iran



نشانی اینترنتی http://nmj.mums.ac.ir/article_10054.html
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