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Iranian Journal of Pathology، جلد ۱۲، شماره ۴، صفحات ۳۷۱-۳۷۶
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عنوان انگلیسی A Comparative Evaluation of ELISA, PCR, and Serum Agglutination Tests For Diagnosis of Brucella Using Human Serum
چکیده انگلیسی مقاله Background & Objective: Since the symptoms of Brucellosis are often atypical and nonspecific, using clinical signs alone to diagnose brucellosis is not advised; therefore, the diagnosis relies predominantly on laboratory testing. Currently, molecular, serological, and microbiological methods are used for diagnosis of this disease. In this study we examined ELISA, PCR and serum agglutination (SAT) methods on human patient serum samples. Methods:A total of 100 serum samples were collected from suspected patients. Fifty serum samples gave a positive result with the Wright test. The ELISA method was first employed on all samples for the detection of IgG and IgM antibodies against Brucella. Subsequently, the rapid PCR methodology was used to identify presence of Brucella genome in 500 µL of each serum sample. The B4/B5 primer pair was used for PCR‌ amplification. Results:Out of the 100 serum samples obtained from patients with suspected brucellosis, 50 samples tested positive by SAT and displayed high titers of 1/160. Of these 50 positive samples, 49 samples were positive as per the ELISA test whereas one sample tested negative. The PCR test was conducted on all 100 serum samples and results showed that the 45 serum samples that gave a positive agglutination test were also positive by PCR. Conclusions: Various laboratory methods have beenused or introduced for the detection of Brucella. Molecular methods such as PCR, a rapid and sensitive method for detection of bacteria, have also been reported. Based on the results of this study, we propose that the simultaneous use of serology and molecular techniques has the potential to overcome limitations of detection thereby enabling the selection of appropriate treatment for the patient.
کلیدواژه‌های انگلیسی مقاله Brucella, PCR, ELISA, Agglutination test, human, Serum
نویسندگان مقاله خشایار محسنی |
student research committee, semnan university of medical sciences, semnan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی سمنان (Semnan university of medical sciences)

رضا میرنژاد |
molecular biology research center, baqiyatallah university of medical sciences, tehran, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی بقیه الله (Baqiyatallah university of medical sciences)

وهاب پیرانفر |
microbiology department, islamic azad university of tonekabon, tonekabon, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی تنکابن (Islamic azad university of tonekabon)

شیوا میرکلانتری |
microbiology department, semnan university of medical sciences, semnan, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی سمنان (Semnan university of medical sciences)

نشانی اینترنتی http://ijp.iranpath.org/article_28293_1000f462dfd346d6075f579a7d7cdc19.pdf
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