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Iranian Journal of Biotechnology، جلد ۱۵، شماره ۴، صفحات ۲۶۰-۲۶۷
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عنوان انگلیسی Sugarcane Mosaic Virus-Based Gene Silencing in Nicotiana benthamiana
چکیده انگلیسی مقاله Background:Potyvirus-based virus-induced gene silencing (VIGS) is used for knocking down the expression of a target gene in numerous plant species. Sugarcane mosaic virus (SCMV) is a monopartite, positive single strand RNA virus. Objectives:pBINTRA6 vector was modified by inserting a gene segment of SCMV in place of Tobacco rattle virus (TRV) genome part 1 (TRV1 or RNA1) and the two nonstructural proteins of TRV2(RNA2). Materials and Methods:SCMV construct was inoculated into 3-4 weeks Nicotiana benthamiana plant leaves either by using a needleless syringe or applying pricking with a toothpick. Results:The construct (SCMV-RNA2) successfully induced post-transcriptional gene silencing (PTGS) of the target genes GFP and ChlI through agroinoculation proving that SCMV is a substitute of the RNA1, which plays a pivotal role in the systemic gene silencing. 2-3-weeks of post inoculation, target genes' silencing was observed in the newly developed non-inoculated leaves. Conclusions:The newly developed construct expresses the knocked down of the endogenous as well as exogenous genes and only four weeks are required for the transient expression of the gene silencing based on SCMV-VIGS system.  
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نویسندگان مقاله ساجد علی |
department of biotechnology, school of sciences, university of management and technology, sialkot campus, sialkot, pakistan

idrees احمد نصیر | idrees ahmad
centre of excellence in molecular biology, university of the punjab, lahore, pakistan

محمد رافق |
institute of clinical psychology, university of management and technology, lahore, pakistan

شهید جاوید butt | shahid javed
department of biotechnology, school of sciences, university of management and technology, sialkot campus, sialkot, pakistan

فاروق ihsan |
centre of excellence in molecular biology, university of the punjab, lahore, pakistan

بینتی qayyum رایو | abdul qayyum
centre of excellence in molecular biology, university of the punjab, lahore, pakistan

طیب husnain |
centre of excellence in molecular biology, university of the punjab, lahore, pakistan

نشانی اینترنتی http://www.ijbiotech.com/article_54473_42d6148c8c320e1312ca15031d9bf749.pdf
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/441/article-441-555723.pdf
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زبان مقاله منتشر شده en
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