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Iranian Journal of Basic Medical Sciences، جلد ۲۱، شماره ۳، صفحات ۲۹۲-۳۰۰
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چکیده فارسی مقاله
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عنوان انگلیسی MiR-103 alleviates autophagy and apoptosis by regulating SOX2 in LPS-injured PC12 cells and SCI rats
چکیده انگلیسی مقاله Objective(s): Recent studies revealed that microRNAs (miRNAs) may play crucial roles in the responses and pathologic processes of spinal cord injury (SCI). This study aimed to investigate the effect and the molecular basis of miR-103 on LPS-induced injuries in PC12 cells in vitro and SCI rats in vivo. Materials and Methods: PC12 cells were exposed to LPS to induce cell injuries to mimic the in vitro model of SCI. The expression of miR-103 and SOX2 in PC12 cells were altered by transient transfections. Cell viability and apoptotic cell rate were measured by CCK-8 assay and flow cytometry assay. Furthermore, Western blot analysis was performed to detect the expression levels of apoptosis- and autophagy- related proteins, MAPK/ERK pathway- and JAK/STAT pathway-related proteins. In addition, we also assessed the effect of miR-103 agomir on SCI rats. Results: LPS exposure induced cell injuries in PC12 cells. miR-103 overexpression significantly increased cell viability, reduced cell apoptosis and autophagy, and opposite results were observed in miR-103 inhibition. miR-103 attenuated LPS-induced injuries by indirect upregulation of SOX2. SOX2 overexpression protected PC12 cells against LPS-induced injuries, while SOX2 inhibition expedited LPS-induced cell injuries. Furthermore, miR-103 overexpression inhibited MAPK/ERK pathway and JAK/STAT pathway through upregulation of SOX2. We also found that miR-103 agomir inhibited cell apoptosis and autophagy in SCI rats. Conclusion: This study demonstrates that miR-103 may represent a protective effect against cell apoptosis and autophagy in LPS-injured PC12 cells and SCI rats by upregulation of SOX2 expression.
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نویسندگان مقاله guowei li | guowei li
department of spine surgery, the fifth affiliated hospital of sun yat-sen university, zhuhai, guangdong 519000, china


تایو chen | tao chen
department of spine surgery, the fifth affiliated hospital of sun yat-sen university, zhuhai, guangdong 519000, china


yingxian zhu | yingxian zhu
department of anaesthesiology, the fifth affiliated hospital of sun yat-sen university, zhuhai, guangdong 519000, china


xiaoyu محمد بن همام رییس کنفدراسیون | xiaoyu xiao
department of anaesthesiology, the fifth affiliated hospital of sun yat-sen university, zhuhai, guangdong 519000, china


juyuan bu | juyuan bu
department of gastroenterological surgery, the fifth affiliated hospital of sun yat-sen university, zhuhai, guangdong 519000, china


zongwen هوانگ | zongwen huang
department of spine surgery, the fifth affiliated hospital of sun yat-sen university, zhuhai, guangdong 519000, china
نشانی اینترنتی http://ijbms.mums.ac.ir/article_10146.html
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-565990.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
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