این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Acta Medica Iranica، جلد ۵۶، شماره ۱، صفحات ۲۸-۳۳
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Wharton's Jelly Derived-Mesenchymal Stem Cells: Isolation and Characterization
چکیده انگلیسی مقاله Wharton`s jelly-derived mesenchymal stem cells (WJ-MSCs), have a high proliferation valency and they do not produce teratogen or carcinogen after subsequent transplantation. They are known as regenerative medicine. Thus more research is needed on the isolation and characterization of mesenchymal stem cells. In this experimental study, we obtained Wharton's jelly tissues from mothers during normal vaginal delivery, after obtaining their informed consent. Mesenchymal stem cells were isolated from cultured Wharton`s jelly, cultured, and were then examined for their proliferation, immunophenotypes, and differentiation capacities. The immunophenotypes of WJ-MSCs were analyzed by flow cytometry. Differentiation was performed resulting in osteogenic, chondrogenic and adipogenic cells. WJ-MSCs formed a homogenous monolayer of adherent spindle-shaped cells. Our results showed the high capacity of the proliferation of WJ-MSCs. Immunophenotyping further confirmed the purity of the isolated cells; their surface antigen expression showed the phenotypical properties like those of WJ-MSCs. The expanded cells were positive for CD 90, CD105, and CD44; they were negative for CD34 and HLA-DR surface markers. The cells had the adipocytic, osteocytic and chondrogenic differentiation capacity. The isolation and characterization of WJ-MSCs with high purity had been conducted, and the results were obtained in a short span. The present study has revealed the feasibility of the culture medium with high glucose and 15% FBS in isolation and proliferation of WJ-MSCs. When Wharton`s jelly pieces were put in the dry bottom of the flask, very effective separation of the MSCs was achieved.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله حسین رنجبران | hossein ranjbaran
immunogenetics research center, school of medicine, mazandaran university of medical sciences, mazandaran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)

سعید عابدیان کناری | saeid abediankenari
immunogenetics research center, school of medicine, mazandaran university of medical sciences, mazandaran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)

مسعود محمدی | masoud mohammadi
department of biostatistics and community medicine, school of medicine, mazandaran university of medical sciences, mazandaran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)

نرجس جعفری | narjes jafari
department of biostatistics and community medicine, school of medicine, mazandaran university of medical sciences, mazandaran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)

علیرضا خلیلیان | alireza khalilian
department of obstetrics, school of medicine, mazandaran university of medical sciences, mazandaran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی مازندران (Mazandaran university of medical sciences)

زهرا رحمانی | zahra rahmani
school of traditional medicine, iran university of medical sciences, tehran, iran.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

مرضیه مومنی نژاد امیری | marzei momeninezhad amiri
school of traditional medicine, iran university of medical sciences, tehran, iran. and department of molecular medicine, sapienza university, rome, italy.

سازمان اصلی تایید شده: دانشگاه علوم پزشکی ایران (Iran university of medical sciences)

پیروز ابراهیمی | pirooz ebrahimi


نشانی اینترنتی http://acta.tums.ac.ir/index.php/acta/article/view/6567
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/56/article-56-567295.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Articles
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات