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Research in Pharmaceutical Sciences، جلد ۹، شماره ۴، صفحات ۲۸۷-۰
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عنوان انگلیسی Effects of disulfiram on apoptosis in PANC-1 human pancreatic cancer cell line
چکیده انگلیسی مقاله Pancreatic carcinoma is currently considered as a rapidly progressive and fatal disease, and is typically diagnosed late in its natural course. It is characterized by a poor diagnosis and lack of response to conventional therapy. Recent studies have suggested that disulfiram (DSF), a member of the dithiocarbamate family, may have antitumor activity. This study aimed to evaluate the in vitro effect of DSF on apoptosis in human pancreatic cancerous cell line (PANC-1). PANC-1 cells were cultured and treated with DSF at doses of 5, 10, 13 µM for 24 h and apoptosis was measured. Methylation specific PCR (MS-PCR) and real-time quantitative PCR were carried out to detect the methylation pattern and to estimate the mRNA expression levels of RASSF1A, p21 and Bax. MS-PCR analysis demonstrated that no unmethylated band was apeared in PANC-1 cell line after DSF treatments. The real-time quantitative PCR results showed no significant mRNA expression for RASSF1A ( p> 0.05); whereas p21 and Bax expression were significantly ( p< 0.01) enhanced after treatment with DSF. The results of the current study indicated that DSF can induce appoptosis in PANC-1 through p21 and Bax pathway but not through RASSF1A.
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نشانی اینترنتی http://rps.mui.ac.ir/index.php/jrps/article/view/1571
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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