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Iranian Journal of Microbiology، جلد ۱۰، شماره ۱، صفحات ۶۵-۷۱
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Seroprevalence and genotyping of avian infectious bronchitis virus detected from Iranian unvaccinated backyard chickens
چکیده انگلیسی مقاله Background and Objectives: Different epidemiological studies have found that backyard chickens are a reservoir for poultry diseases. Most backyard chicken flocks have a poor level of biosecurity, which increases the risk of spread of diseases. In recent years, the number of backyard chickens has been on the rise in Iran. However, the health status of backyard flocks is still poorly documented. Thus, this study aimed at examining the seroprevalence of antibodies against infectious bronchitis virus (IBV) and molecular surveillance and genotyping of IBV among backyard chickens (without vaccination history) in Mazandaran province, North of Iran, 2014. Materials and Methods: A total of 460 blood samples of unvaccinated backyard chickens in the mentioned area were tested for antibodies against IBV using commercial ELISA. Also, cecal tonsils were collected from 75 chickens in the same area. Real time RT-PCR (for detection) and RT-PCR and sequencing spike gene were performed. Results: The seropositivity rate was 54.5%. In addition, we detected 793/B, Variant 2, and QX in the backyard flocks and performed phylogenetic studies on them. The phylogenetic study revealed that the detected genotypes had high homology with IBV strains that were infected broilers, pullets, and layers in Iran. Conclusion: There is a need for continuous monitoring of IBV among avian species to complete the epidemiological map and work on the pathogenesis of Iranian IBV strains in Iranian backyard chickens.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله شیما شکری | shima shokri
department of clinical sciences, faculty of veterinary medicine, university of tehran, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

وحید کریمی | vahid karimi
department of microbiology and immunology, faculty of veterinary medicine, university of tehran, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

آرش قلیانچی لنگرودی | arash ghalyanchi langeroudi
department of clinical sciences, faculty of veterinary medicine, university of tehran, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

مهدی وصفی مرندی | mehdi vasfi marandi
razi vaccine and serum research institute, agricultural research and extension organization, tehran, iran

سازمان اصلی تایید شده: موسسه تحقیقات واکسن و سرم سازی رازی (Razi vaccine and serum research institute)

مسعود هاشم زاده | masoud hashemzadeh
department of clinical sciences, faculty of veterinary medicine, university of tehran, tehran, iran

سازمان اصلی تایید شده: دانشگاه تهران (Tehran university)

طاها zabihipetroudi | taha zabihipetroudi
department of pathobiology, school of veterinary medicine, shiraz university, shiraz, iran

سازمان اصلی تایید شده: دانشگاه شیراز (Shiraz university)

حمیده نجفی | hamideh najafi
department of health and management of poultry diseases, iranian veterinary organization, tehran, iran


فرشاد تهرانی | farshad tehrani


نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/1211
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/91/article-91-573595.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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