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Medical Laboratory Journal، جلد ۱۲، شماره ۲، صفحات ۰-۰
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عنوان انگلیسی Isolation and molecular identification of keratinase-producing bacteria from the sludge of Qeshm Island
چکیده انگلیسی مقاله ABSTRACT: Keratinase enzyme in the production of detergents, cosmetics, pharmaceuticals, leather industry, and dietary supplements production is used by livestock, and also in newer fields such as the analysis of prions for Bovine Spongiform Encephalopathy (BSE) treatment is used. Since the use of traditional methods for decomposition of feathers, hair, hooves, nails, and wool that contain high levels of keratin, is costly and leads to elimination of amino acids, biodegrading of the mentioned cases by bacterial keratinase can be a valuable solution. The aim of this study was, isolation and molecular identification of bacteria in Qeshm Island and Peyposht village that were able to break down keratin. For this purpose, water and sludge samples from Qeshm Island and Peyposht village were collected. After enrichment of bacteria, screening enzyme-producing species keratinases was performed for isolates by Lowry method, in the next phase, the effect of pH elements and temperature on production of keratinase enzyme as well as the growth of microorganisms was examined, and then for molecular identification of the isolates, colony-PCR method, were used. Bacillus berevis and Enterobacter cloacae bacteria were isolated in this study. Keratinase enzyme derived from Bacillus berevis at pH=7.5 and a temperature of 35 ° C and keratinase enzyme derived from Enterobacter cloacae at pH=7 and 37 ° C temperature showed the highest keratinase activity. By considering the great needs of the country in various industries to industrial enzymes, it seems that the use of local strains can be effective in making the country needless import this product. Keywords: Keratinolytic protein, Colony PCR, Bacteria, Identification, Sewage  
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نویسندگان مقاله | Maedeh Kiani Abri
Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran


| Monir Doudi
Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran


| Ahadi Ali Mohammad
Department of Genetics, Shahrekord University Branch, Shahrekord, Iran
نشانی اینترنتی http://goums.ac.ir/mljgoums/browse.php?a_code=A-10-1-510&slc_lang=fa&sid=en
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زبان مقاله منتشر شده fa
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