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Physiology and Pharmacology، جلد ۱۲، شماره ۲، صفحات ۱۰۹-۱۱۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Expression and Purification of the luciferase enzyme and in Vivo ATP Assay
چکیده انگلیسی مقاله Introduction: Gene expression and purification of luciferases from the firefly, Lampyris turkestanicus, and optimization of cellular ATP measurements were performed. Methods: cDNA encoding luciferases from Lampyris turkestanicus was transferred from pQE30 vector into pET28a expression vector and pLtu28 was built. Newly constructed vector was expressed in E. coli XL1 Blue and the recombinant luciferase was purified using Ni-NTA Sepharose column. Enzymatic properties (Km and Vmax) for ATP were measured using luminescence assay. Standard curve of ATP was obtained by Promega ATP detection kit and designed method based on the L. turkestanicus luciferase and ATP serial dilution. Moreover bacterial ATP was measured by Promega kit and designed method using L. turkestanicus luciferase. Results: Results showed that ligation of L. turkestanicus luciferase encoding cDNA into pET28a and transformation of recombinant vector into competent cells was performed efficiently. Using luciferin, positive colonies were screened and cultured. SDA-PAGE showed that recombinant luciferase was efficiently purified by Ni-NTA Sepharose column. ATP standard curve and measurement of bacteria, using Promega and designed method by L. turkestanicus luciferases showed high identity. Conclusion: comparison of developed assay with promega kits in identification of bacterial concentration show its high quality and potent ability in ATP detection.
کلیدواژه‌های انگلیسی مقاله Bioluminescence, Lampyris turkestanicus, Luciferase, ATP.
نویسندگان مقاله سامان حسین خانی | saman hosseinkhani
biochemistry department, faculty of basic sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

مجتبی مرتضوی | mojtaba mortazavi
biochemistry department, faculty of basic sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

aboarhman امامزاده | aboarhman emamzadeh
biochemistry department, faculty of basic sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

نشانی اینترنتی http://www.phypha.ir/ppj/browse.php?a_code=A-10-223-1&slc_lang=en&sid=en
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Research
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