این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Physiology and Pharmacology، جلد ۱۱، شماره ۳، صفحات ۱۹۲-۱۹۸
عنوان فارسی Cholinergic Differentiation of neural precursor cells derived from mouse embryonic stem cells increased by Shh, LIF and RA
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Cholinergic Differentiation of neural precursor cells derived from mouse embryonic stem cells increased by Shh, LIF and RA
چکیده انگلیسی مقاله Introduction Cholinergic system is one of the important systems of mammalian CNS. Cholinergic neurons distributed in brain and spinal cord and contributed to principal functions like: consciousness, learning and memory, and motor control. In this study we investigated the differentiation potentiality of mouse embryonic stem cells toward cholinergic neurons. The aim of this study was to evaluate the effect of sonic hedgehog (Shh), Retinoic Acid (RA), LIF, IL6 and NGF on differentiation of neural progenitor cells (NPCs), produced by lineage selection method, to cholinergic neurons. Material and methods Royan B1, mouse embryonic stem cells derived from C57BL/6 strain was used to produce aggregates. Aggregates were cultured in serum free medium to produce nestin positive or NPCs, then cell expansion was achieved by treatment with EGF and FGF2. Following withdrawal of EGF and FGF2, the cells were further cultured in presence or absence of differentiation factors in serum containing medium. Relative number of neurons and cholinergic neurons were assessed by immunohistochemical procedure using antibodies against MAP2, B-tubulin3, and ChAT. Results Data obtained show that around 70% of cells were B-tubulin3 positive. We found ChAT immunoreactivity in cultured cells in both treated and control groups. Conclusion This study shows that some of the neurons produced by lineage selection method are cholinergic neurons, and the percentage of cholinergic neurons increased after treatment by Shh, LIF and RA.
کلیدواژه‌های انگلیسی مقاله Embryonic stem cells, Neuron, Cholinergic, Differentiation
نویسندگان مقاله فرشاد همایونی مقدم | farshad homayouni moghadam
department of physiology, medical faculty, isfahan university of medical science, isfahan, iran

سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

hojatoallah علایی | hojatoallah alaie
department of physiology, medical faculty, isfahan university of medical science, isfahan, iran

سازمان اصلی تایید شده: دانشگاه اصفهان (Isfahan university)

خدیجه کربلایی | khadije karbalaie
department of stem cell, royan institute, isfahann campus, isfahan, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

سمیه تنهایی | somayeh tanhaei
department of stem cell, royan institute, isfahann campus, isfahan, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

محمد حسین نصر اصفهانی | mohammad hossein nasr esfahani
department of stem cell, royan institute, isfahann campus, isfahan, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

حسین بهاروند | hossein baharvand
department of stem cell, royan institute, tehran, iran

سازمان اصلی تایید شده: پژوهشگاه رویان (Royan institute)

نشانی اینترنتی http://www.phypha.ir/ppj/browse.php?a_code=A-10-146-1&slc_lang=en&sid=en
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده Neurodegeneretive diseases
نوع مقاله منتشر شده Original Research
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات