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Research in Pharmaceutical Sciences، جلد ۱۳، شماره ۳، صفحات ۲۲۲-۲۳۸
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چکیده فارسی مقاله
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عنوان انگلیسی Bioprocess and downstream optimization of recombinant human growth hormone in Pichia pastoris
چکیده انگلیسی مقاله The methylotrophic yeast Pichia pastoris is a well-established expression host, which is often used in the production of protein pharmaceuticals. This work aimed to evaluate the effect of various concentrations of ascorbic acid in mixed feeding strategy with sorbitol/methanol on productivity of recombinant human growth hormone (r-hGH). The relevant concentration of ascorbic acid (5, 10, or 20 mmol) and 50 g/L sorbitol were added in batch-wise mode to the medium at the beginning of induction phase. The rate of methanol addition was increased stepwise during the first 12 h of production and then kept constant. Total protein and r-hGH concentrations were analyzed and the results compared with sorbitol/methanol feeding using one-way analysis of variance. Moreover, an effective clarification process using activated carbon was developed to remove process contaminants like pigments and endotoxins. Finally, a three-step chromatographic process was applied to purify the product. According to the obtained results, addition of 10 mmol ascorbic acid to sorbitol/methanol co-feeding could significantly increase cell biomass (1.7 fold), total protein (1.14 fold), and r-hGH concentration (1.43 fold). One percent activated carbon could significantly decrease pigments and endotoxins without any significant changes in r-hGH assay. The result of the study concluded that ascorbic acid in combination with sorbitol could effectively enhance the productivity of r-hGH. This study also demonstrated that activated carbon clarification is a simple method for efficient removal of endotoxin and pigment in production of recombinant protein in the yeast expression system. 
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Saeed Azadi
2Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, I.R. Iran.


| Seyed Kazem Sadjady
3School of Pharmacy, Shahid Beheshti University of Medical Sciences, Tehran, I.R. Iran.


| Seyed Alireza Mortazavi
4Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, I.R. Iran.


| Nasser Naghdi
5Food Safety Research Center, Shahid Beheshti University of Medical Sciences, Tehran, I.R. Iran.


| Arash Mahboubi
2Pharmaceutical Sciences Branch, Islamic Azad University, Tehran, I.R. Iran.


| Roya Solaimanian


نشانی اینترنتی http://rps.mui.ac.ir/index.php/jrps/article/view/1827
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/115/article-115-628429.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
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