این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Basic Medical Sciences، جلد ۲۱، شماره ۷، صفحات ۶۷۸-۶۸۱
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی AGS cell line xenograft tumor as a suitable gastric adenocarcinoma model: growth kinetic characterization and immunohistochemistry analysis
چکیده انگلیسی مقاله Objective(s): Gastric cancer is the third leading cause of cancer-related death worldwide. The overall survival rate of patients is poor because gastric cancers are usually diagnosed at the late stages. Therefore, further research is needed and appropriate research tools are required to develop novel therapeutic approaches.Materials and Methods: Eight female athymic nude mice with a C57BL/6 background were used in this study. AGS cells were inoculated into the flank. The tumor volumes were calculated and growth curves were drawn. When the volume of the tumors reached 1000 mm3, the animals were humanely euthanized with CO2 gas. After harvesting, tumors were analyzed with Hematoxylin and Eosin (H&E) and immunohistochemistry (IHC). A pathologist confirmed tumor entity through H&E staining. Tumors were evaluated for expression of HER-2, P53, Ki-67, CD34, cytokeratin 8 (CK8), vimentin, estrogen receptor (ER), and progesterone receptor (PR) utilizing immunohistochemistry.Results: The tumor take rate was 62.5%, mean doubling time was 40.984 d, and the latency period was 30.62 days. The H&E staining results showed highly malignant hyperchromatin epithelial cells. IHC assessment showed the mutation status of P53 gene. The expression score of the CK8 protein in the tumor cells was +3. Vimentin protein was not expressed and changes in mesenchymal phenotype were not observed. Ki-67 IHC indicated that the proliferation rate was >43% and angiogenesis was defined as high MVD.Conclusion: The respective AGS xenograft model provides an opportunity to understand the pattern of tumor growth as well as to evaluate new gastric cancer therapies in in vivo studies.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Tahereh Barati
Cancer Biology Research Center, Tehran University of Medical Sciences, Tehran, Iran


| Mahnaz Haddadi
Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran


| Fatemeh Sadeghi
Cancer Biology Research Center, Tehran University of Medical Sciences, Tehran, Iran


| Samad Muhammadnejad
Research Center for Molecular and Cellular Imaging, Tehran University of Medical Sciences, Tehran, Iran


| Ahad Muhammadnejad
Vali-e-Asr Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran


| Ronak Heidarian
Vali-e-Asr Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran


| Motahareh Arjomandnejad
Vali-e-Asr Reproductive Health Research Center, Tehran University of Medical Sciences, Tehran, Iran


| Saeid Amanpour
Cancer Biology Research Center, Tehran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی http://ijbms.mums.ac.ir/article_10753.html
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/87/article-87-639357.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات