این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Microbiology، جلد ۱۰، شماره ۲، صفحات ۹۸-۱۰۳
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی First detection of New Delhi metallo-β-lactamases variants (NDM-1, NDM-2) among Pseudomonas aeruginosa isolated from Iraqi hospitals
چکیده انگلیسی مقاله Background and Objectives: Multidrug resistance and in particular, carbapenem resistant Gram-negative bacteria is spreading worldwide at an alarming rate. Among the clinically significant carbapenemases, the New Delhi Metallo-β-lactamase (NDM) is one of the most formidable. NDM efficiently hydrolyses β-lactams and is the last-resort among carbapenems. Hence, therapeutic options for NDM producer bacteria become restricted to a handful of antibiotics. The present study was undertaken to detect the prevalence of the bla NDM-variants Metallo β- lactamases (MBLs) among isolates of Pseudomonas aeruginosa recovered from various clinical samples of hospitalized patients in Baghdad, Iraq. Materials and Methods: A total of 100 isolates of Gram-negative bacteria obtained from various clinical samples were subjected to antibiotic susceptibility testing by the disc-diffusion method against meropenem (10 µg), imipenem (10 µg), doripenem (10 µg), polymyxin B (10 µg), colistin (10 µg), amikacin (30 µg), gentamicin (10 µg), aztreonam (30 µg), ciprofloxacin (5 µg), levofloxacin (5 µg), ofloxacin (5 µg), cefepime (30 µg), ceftazidime (30 µg), piperacillin-tazobactam (10010 µg), tigecycline (15 µg) and tetracycline (10 µg). The results were interpreted according to the guidelines suggested by the Clinical Laboratory Standards Institute. Presence of bla NDM was detected by PCR and it was confirmed by DNA sequencing of the gene present in the isolates that exhibited carbapenem resistance. Results: In the present study, four isolates of P. aeruginosa carried the bla NDM, three isolates harboured bla NDM-1 and one isolate harboured bla NDM-2. All isolates were resistant to imipenem and meropenem. The bla NDM-1 carrying isolates remained susceptible to colistin and β-lactamase inhibitors piperacillin-tazobactam. Conclusion: We are reporting emergence of the P. aeruginosa carrying the bla NDM-variant, which exhibited resistance to imipenem and meropenem for the first time in Iraq.
کلیدواژه‌های انگلیسی مقاله P. aeruginosa, Carbapenem resistance, blaNDM variants 1, 2
نویسندگان مقاله | Sarah Jehad Ismail
Department of Biotechnology, University of Baghdad, AL Mansour City, Baghdad, Iraq


| Suhad Saad Mahmoud


نشانی اینترنتی http://ijm.tums.ac.ir/index.php/ijm/article/view/1642
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/91/article-91-639381.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article(s)
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات