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Iranian Biomedical Journal، جلد ۲۰، شماره ۱، صفحات ۱۲-۱۷
عنوان فارسی in vitro Anti-Proliferative Effect of Adiponectin on Human Endometriotic Stromal Cells through AdipoR۱ and AdipoR۲ Gene Receptor Expression
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عنوان انگلیسی in vitro Anti-Proliferative Effect of Adiponectin on Human Endometriotic Stromal Cells through AdipoR1 and AdipoR2 Gene Receptor Expression
چکیده انگلیسی مقاله Background: Endometriosis is a complex disorder in reproductive age women which consist of stromal and epithelial cells implantation outside the uterine cavity. Adiponectin is a member of cytokine family with various metabolic roles and proliferation inhibition of many cancer cells. The aim of the present research was to determine adiponectin effect on human endometriotic stromal cells (ESCs) proliferation and their expression of adiponectin receptors. Methods: In this experimental study, endometrial biopsies (n = 7) were taken. ESCs isolation was done by enzymatic digestion and cell filtrations. ESCs of each biopsy were divided into four groups: 0 (control), 10, 100, and 200 ng/ml adiponectin concentrations in three different times (24, 48 or 72 h). The effect of adiponectin on ESC viability and expression of mRNA Adipo receptor1 (R1) and Adipo receptor2 (R2) was determined by Trypan blue staining and semi-quantitative RT-PCR, respectively. Data were analyzed by one-way ANOVA and unpaired student's t-test, and P < 0.05 was considered statistically significant. Results: Adiponectin inhibited human endometriotic stromal cell proliferation in time- and dose-dependent manners significantly (P = 0.001). Expression of AdipoR1 and AdipoR2 gene receptors was increased in human ESCs significantly (P < 0.05). Conclusions: Adiponectin can suppress endometriosis by inhibiting ESC proliferation and increased AdipoR1 and AdipoR2 expression.
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نویسندگان مقاله سمیه بهلولی | somayeh bohlouli


آرزو rabzia | arezou rabzia


احسان صادقی | ehsan sadeghi


فرزانه چوبساز | farzaneh chobsaz


مظفر خزایی | mozafar khazaei


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-579&slc_lang=en&sid=en
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