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Iranian Biomedical Journal، جلد ۱۹، شماره ۴، صفحات ۱۸۸-۱۹۳
عنوان فارسی Expression Analysis of mir-۲۱ and mir-۲۲۱ in Cancerous Tissues from Iranian Patients with Gastric Cancer
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Expression Analysis of mir-21 and mir-221 in Cancerous Tissues from Iranian Patients with Gastric Cancer
چکیده انگلیسی مقاله Background: Early detection is a key to survival for gastric cancer. Molecular markers such as miRNA (microRNA) can have great importance in the early diagnosis of gastric cancer. Expression of miR-21 and miR-221 are deregulated in many types of human cancers. This study aimed to investigate the differences in miRNA expression patterns within the Iranian population. Methods: Total RNA was extracted from gastric cancer tissues and adjacent non-cancerous tissues from 32 patients. Expression levels of miR-21 and miR-221 were detected by Real time RT-PCR using a specific primer, with 5s rRNA as the internal reference gene. Results: Our data showed that the expression levels of miR-21 and miR-221 in gastric cancer samples were significantly higher than in paired non-cancerous samples (P < 0.05). The receiver operating characteristic (ROC) analyses yielded the area under the curve (AUC) values of 80.30 for miR-21 and 93.30 for miR-221, and combined ROC analysis revealed the highest AUC value of 96.90 in discriminating GC patients from healthy controls. Conclusion: It seems that miR-21 and miR-221 expression pattern in Iranian patients with gastric cancer are similar to any other population. Considering the increased expression level of two miRNAs in cancerous tissue compared to normal tissue as well as the area under ROC curve, miR-21 and miR-221 can be used for early detection of gastric cancer.
کلیدواژه‌های انگلیسی مقاله MicroRNAs, Tumor markers, Stomach neoplasms
نویسندگان مقاله حسین عفت پناه | hosein effatpanah


رضا یادگار آذری | reza yadegarazari


منوچهر کرمی | manoochehr karami


امیر مجلسی | amir majlesi


نوشین شباب | nooshin shabab


مسعود سعیدی جم | massoud saidijam


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-564&slc_lang=fa&sid=en
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زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده Related Fields
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