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Iranian Biomedical Journal، جلد ۱۹، شماره ۴، صفحات ۱۹۴-۲۰۵
عنوان فارسی Effects of Peptone Supplementation in Different Culture Media on Growth, Metabolic Pathway and Productivity of CHO DG۴۴ Cells a New Insight into Amino Acid Profiles
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عنوان انگلیسی Effects of Peptone Supplementation in Different Culture Media on Growth, Metabolic Pathway and Productivity of CHO DG44 Cells a New Insight into Amino Acid Profiles
چکیده انگلیسی مقاله Background: The optimization of bioprocess conditions towards improved growth profile and productivity yield is considered of great importance in biopharmaceutical manufacturing. Peptones as efficient sources of nutrients have been studied for their effect on media development however, their role on metabolic pathway is not well understood. Methods: In the present study, the effect of different concentration of peptones on a recombinant Chinese hamster ovary (CHO) cell line grown in three serum-free suspension cultures was determined. Six peptones of different origins and available amino acid profiles were investigated regarding their impact on cell growth, productivity, and metabolic pathways changes. Results: In optimized feeding strategies, increases of 136% and 159% in volumetric productivity (for a low-nutrient culture media) and 55% (for a high-nutrient culture media) were achieved. Furthermore, particular sources of peptones with specific amino acid profile developed preferential results for each different culture medium. Two peptones, SoyA2SC and SoyE-110, were the only hydrolysates that showed production improvement in all three media. Casein Peptone plus Tryptone N1 and SoyA3SC showed different improved results based on their implemented concentration for each individual basal medium. Conclusion: The amino acid profile of peptones may provide clues to identify the most effective feeding strategies for recombinant CHO cells.
کلیدواژه‌های انگلیسی مقاله CHO cells, Culture media, Peptones, Recombinant proteins
نویسندگان مقاله فاطمه دوامی | fatemeh davami


فرناز اقبال پور | farnaz eghbalpour


لیلا نعمت اللهی | leila nematollahi


فرزانه برخورداری | farzaneh barkhordari


فریدون مهبودی | fereidoun mahboudi


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-568&slc_lang=en&sid=en
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زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده Related Fields
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