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Iranian Biomedical Journal، جلد ۱۹، شماره ۲، صفحات ۸۲-۹۰
عنوان فارسی ارزیابی توان تولید سلول نرونی سلولهای مزانشیمال بندناف انسان: یک روش وابسته به غلظت و زمان
چکیده فارسی مقاله
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عنوان انگلیسی Evaluation of Neurogenic Potential of Human Umbilical Cord Mesenchymal Cells a Time- and Concentration- Dependent Manner
چکیده انگلیسی مقاله Background: Retinoic acid as one of the most important regulators for cell differentiation was examined in this study for differentiation of human umbilical mesenchymal cells (hUCM). Methods: After isolation, hUCM were evaluated for mesenchymal stem cell properties by flow cytometry and alkaline phosphatase assay. Also, doubling time of the cells and their differentiation potential into adipogenic and osteogenic cells were tested. hUCM were then cultured with different concentrations of retinoic acid, and on days 1, 7, and 12, the percentage of differentiated cells was determined by immunostaining for nestin, anti-microtubule associated protein 2 (MAP2), glutamic acid decarboxylase (GAD), and gamma-aminobutyric acid (GABA) markers. Results: The isolated cells were negative for the hematopoietic markers and positive for the mesenchymal markers. They showed the population doubling time 60 ± 3 hours and differentiated into osteogenic and adipogenic cells. A descending trend in nestin and an ascending trend in MAP2, GAD, and GABA expression were observed from the first day until the last day between different concentrations of retinoic acid. Conclusion: hUCM cells may have the potential to differentiate into neural cells in the presence of different incubation period and concentration of retinoic acid.
کلیدواژه‌های انگلیسی مقاله Cell differentiation, Neural stem cells, Retinoic acid
نویسندگان مقاله سید حسن افتخار واقفی | seyed hassan eftekhar vaghefi


لیلا زحمتکش | leila zahmatkesh


پروین صالحی نژاد | parvin salehinejad


شهین توتونچی | shahin totonchi


علی شمس آرا | ali shams ara


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-543&slc_lang=en&sid=en
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زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده Related Fields
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