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Iranian Biomedical Journal، جلد ۱۸، شماره ۱، صفحات ۴۹-۵۴
عنوان فارسی تاثیر همکشتی فیبروبلاست و Activin A بر رشد آزمایشگاهی فولیکول های پره آنترال موش
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عنوان انگلیسی The Effects of Fibroblast Co-Culture and Activin A on in vitro Growth of Mouse Preantral Follicles
چکیده انگلیسی مقاله Background: This study was conducted to evaluate fibroblast co-culture and Activin A on in vitro maturation and fertilization of mouse preantral follicles. Methods: The ovaries from 12-14-day-old mice were dissected, and 120-150 μm preantral follicles were cultured individually in α-MEM as based medium for 12 days. A total number of 456 follicles were cultured in four conditions: (i) base medium as control group (n = 113), (ii) base medium supplemented with 30 ng/ml Activin A (n = 115), (iii) base medium co-cultured with mouse embryonic fibroblast (n = 113), and (iv) base medium supplemented with 30 ng/ml Activin A and co-cultured with fibroblast (n = 115). Rate of growth, survivability, antrum formation, ovulation, embryonic development and steroid production were evaluated. Analysis of Variance and Duncan test were applied for analyzing. Results: Both co-culture and co-culture + Activin A groups showed significant difference (P< 0.05) in growth (on days 4, 6, and 8 of culture period) and survival rates. However, there was no significant difference in antrum formation, ovulation rate, and embryonic development of ovulated oocytes. There were significant differences (P< 0.05) in the estradiol production on days 8, 10, and 12 between co-culture + Activin A and the control group. Progesterone production also was significant (P< 0.05) in co-culture + Activin A group on days 6, 8, 10, and 12 compared to control group. Conclusion: Fibroblast co-culture and Activin A promoted growth and survivability of preantral follicles. However, simultaneous use of them was more efficient.
کلیدواژه‌های انگلیسی مقاله Fibroblast, Activin A, Follicle
نویسندگان مقاله عباسعلی کریمپور ملکشاه | abbasali karimpour malekshah


محمود حیدری | mahmoud heidari


کاظم پریور | kazem parivar


نسرین سادات اعظمی | nasrin sadat azami


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-512&slc_lang=en&sid=en
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