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Iranian Biomedical Journal، جلد ۱۸، شماره ۱، صفحات ۵۵-۵۹
عنوان فارسی مهار ژن sidB با استفاده از RNAi در Aspergillus nidulans
چکیده فارسی مقاله
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عنوان انگلیسی Down-Regulation of sidB Gene by Use of RNA Interference in Aspergillus nidulans
چکیده انگلیسی مقاله Background: Introduction of the RNA interference (RNAi) machinery has guided the researchers to discover the function of essential vital or virulence factor genes in the microorganisms such as fungi. In the filamentous fungus Aspergillus nidulans, the gene sidB plays an essential role in septation, conidiation and vegetative hyphal growth. In the present study, we benefited from the RNAi strategy for down-regulating a vital gene, sidB, in the fungus A. nidulans. Methods: The 21-nucleotide small interfering RNA (siRNA) was designed based on the cDNA sequence of the sidB gene in A. nidulans. Transfection was performed through taking up siRNA from medium by 6 hour-germinated spores. To evaluate the morphologic effects of siRNA on the fungus, germ tube elongation was followed. Moreover, total RNA was extracted and quantitative changes in expression of the sidB gene were analyzed by measuring the cognate sidB mRNA level by use of a quantitative real-time RT-PCR assay. Results: Compared to untreated-siRNA samples, a significant inhibition in germ tube elongation was observed in the presence of 25 nM of siRNA (42 VS 21 µM). In addition, at the concentration of 25 nM, a considerable decrease in sidB gene expression was revealed. Conclusion: Usage of RNAi as a kind of post-transcriptional gene silencing methods is a promising approach for designing new antifungal agents and discovering new drug delivery systems.
کلیدواژه‌های انگلیسی مقاله Aspergillus nidulans, sidB, RNAi
نویسندگان مقاله حمید اسلامی | hamid eslami


محمدرضا خرمی زاده | mohammad reza khorramizadeh


محمدرضا پورمند | mohammad reza pourmand


مریم موذنی | maryam moazeni


ساسان رضایی | sassan rezaie


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-513&slc_lang=en&sid=en
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کد مقاله (doi)
زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده Related Fields
نوع مقاله منتشر شده مقاله کامل
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