این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Biomedical Journal، جلد ۱۷، شماره ۴، صفحات ۱۷۹-۱۸۶
عنوان فارسی انجماد شیشه ای و ذوب بافت تخمدان انسانی تاثیر اندکی بر بیان ژنهای وابسته به آپوپتوز دارد
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Human Ovarian Tissue Vitrification/Warming Has Minor Effect on the Expression of Apoptosis-Related Genes
چکیده انگلیسی مقاله Background: In this study, we evaluated the incidence of apoptosis at the ultrastructural levels and expression of some apoptosis-related genes in vitrified human ovarian tissue just after warming. Methods: Human ovarian tissue biopsies from 23 women after caesarean section were transported to the laboratory within 2 hours, and then they were cut into small pieces. Some pieces were vitrified and warmed and the other samples were considered as control. Apoptosis was assessed by a transmission electron microscope and also by molecular analysis of pro-apoptotic (Fas, FasL, Bax, p53, caspase8, and caspase3) and antiapoptotic (Bcl-2 and BIRC5) genem RNA levels using real-time RT-PCR before and after vitrification. Results: No sign of apoptosis was shown ultrastructurally in vitrified samples. The level of FasL, Bcl-2, Bax, p53, and caspase3 mRNA and Bax:Bcl-2 ratio were similar in non-vitrified and vitrified groups however, the expression of Fas and caspase8 genes was higher and BIRC5 was lower in vitrified samples compared to non-vitrified group (P< 0.05). Conclusion: The fine structure of human vitrified ovarian tissue was well preserved moreover, vitrification was shown to affect the expression of some apoptosis-related genes. However, additional study is needed to confirm this observation.
کلیدواژه‌های انگلیسی مقاله Vitrification, Apoptosis, Gene expression, Ovary, Humans
نویسندگان مقاله معصومه عبدالهی | maasoume abdollahi


مژده صالح نیا | mojdeh salehnia


ساغر صالح پور | saghar salehpour


نسیم قربانمهر | nassim ghorbanmehr


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-485&slc_lang=en&sid=en
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده Related Fields
نوع مقاله منتشر شده مقاله کامل
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات