این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Biomedical Journal، جلد ۱۴، شماره ۴، صفحات ۱۲۷-۱۳۵
عنوان فارسی hsp۹۰α and hsp۹۰β Expression during Xenopus laevis Embryonic Development
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Expression of hsp90 Alpha and hsp90 Beta during Xenopus laevis Embryonic Development
چکیده انگلیسی مقاله Background: Members of the eukaryotic Hsp90 family function as important molecular chaperones in the assembly, folding and activation of cellular signaling in development. Two hsp90 genes, hsp90 alpha and hsp90 beta, have been identified in fish and homeothermic vertebrates but not in poikilothermic vertebrates. In the present study, the expression of hsp90 alpha and hsp90 beta genes in Xenopus laevis, which is phylogenetically positioned between zebrafish and mammals, has been addressed. Methods: Partial Xenopus hsp90 alpha and hsp90 beta cDNA were identified and isolated using RT-PCR, and a full-length Xenopus hsp90 beta cDNA was isolated from an embryonic cDNA library. Northern-blot analysis was used to study the expression of hsp90 alpha and hsp90 beta genes in total RNA of the embryos and in situ hybridization was used to compare the expression of these genes with that of hsp70 and MyoD genes in Xenopus embryogenesis. Results: Northern-blot analysis revealed that the hsp90 beta gene was strongly expressed constitutively at all stages of embryogenesis, but weakly induced following the heat shock. In contrast, the hsp90 alpha gene was weakly expressed in embryos at control temperature, but strongly up-regulated following heat shock. In situ hybridization results showed that hsp90 alpha gene was observed predominantly in cells of the developing somite. Microscopic sections showed that hsp90 alpha and MyoD mRNA are expressed in similar regions in somite and this pattern was distinct from that of hsp70 and hsp90 beta. Conclusion: These data support the hypothesis that the presence of hsp90 alpha and hsp90 beta genes is conserved among vertebrates, and these genes are differentially regulated in a tissue, stress, and development stage-specific manner.
کلیدواژه‌های انگلیسی مقاله Xenopus, hsp90 alpha, hsp90 beta
نویسندگان مقاله علی اکبر طاهریان | aliakbar taherian


نیک آوسنک | nick ovsenek


پاتریک کرون | patrick h krone


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-181&slc_lang=en&sid=en
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده Related Fields
نوع مقاله منتشر شده مقاله کامل
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات