این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Biomedical Journal، جلد ۱۴، شماره ۱، صفحات ۹-۱۵
عنوان فارسی Differential Gene-Expression of Metallothionein ۱M and ۱G in Response to Zinc in Sertoli TM۴ Cells
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Differential Gene-Expression of Metallothionein 1M and 1G in Response to Zinc in Sertoli TM4 Cells
چکیده انگلیسی مقاله Background: Zinc (Zn) as an important trace element is essential for testicular development and spermatogenesis. Molecular mechanism of Zn action in the reproductive system may be related to metal binding low-molecular weight proteins, metallothioneins (MT). Our objective was to determine the effect of Zn on two important isoforms of MT, MT1M and MT1G genes expression on testicular sertoli cells. Methods: Cultured sertoli TM4 cells were exposed to different concentrations of Zn at different time points. Cellular uptake of Zn was tested using flame atomic absorption spectrometry. The cellular viability and gene expression were assessed by MTT and real-time PCR methods, respectively. Results: The treated cells resulted in higher Zn concentration and cellular viability. The expression of MT1M and MT1G genes in the treated cells were greater than those of the untreated cells (P< 0.05). In the high dosage treated group (100 and 500 μM), Zn concentration and expression of MT1M and MT1G genes increased three h after treatment MT1G gene expression increased more at sixth h. At 18th h of treatment, the expression of both genes especially MT1G, increased dramatically while Zn concentration decreased. Conclusion: Since the increase of MT1G mRNA was coincident with cellular Zn level, it seems that MT1G has a more prominent role than MT1M in the homeostasis of Zn. In addition, Zn at dosage of 50 μM (pharmacologic concentration) may protect cells by increasing the expression of MT genes at longer periods.
کلیدواژه‌های انگلیسی مقاله Gene expression, Metallothionein (MT), Sertoli cells, Zinc
نویسندگان مقاله فاطمه خردمند | fatemeh kheradmand


عیسی نورمحمدی | issa nourmohammadi


محمد حسین مدرسی | mohammad hossein modarressi


محسن فیروزرای | mohsen firoozrai


محمد امین احمدی فقیه | mohammad amin ahmadi faghih


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-165&slc_lang=en&sid=en
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده Related Fields
نوع مقاله منتشر شده مقاله کامل
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات