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Iranian Biomedical Journal، جلد ۱۳، شماره ۲، صفحات ۱۱۷-۱۲۳
عنوان فارسی الفاء سلول های استرومایی مغز اسنخوان به سلول های شبه کولینرژیک با استفاده از فاکتور رشد عصبی
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عنوان انگلیسی Induction of Bone Marrow Stromal Cells into Cholinergic-Like Cells by Nerve Growth Factor
چکیده انگلیسی مقاله Background: Bone marrow stromal cells (BMSC) are used as a source for cell therapy in different model for neurological disorder such as stroke and spinal cord injury. However, the transdifferentiation of BMSC into cholinergic phenotype requires more investigation. Methods: BMSC were isolated from adult rats, pre-induced with β-mercaptoethanol (BME) and followed by nerve growth factor (NGF) induction. Neurofilaments of 68 kDa, 160 kDa and 200 kDa (NF-200, NF-160 and NF-68, respectively) immuno-staining were used for evaluating the transdifferentiation of BMSC into neuronal phenotype. The percentage of neurofilaments immuno-reactive cells was applied in order to evaluate the results at the pre-induction and the induction stages. Also, NeuroD and Oct-4 expressions, using RT-PCR, were used in assessing the progression of BMSC into neuronal lineage. Choline acetyltransferase immuno-reactive cells were used for estimating the percentage of cholinergic neuronal phenotype. Immuno-staining with anti-microtubule-associated protein-2 (MAP-2) and anti-synapsin-I antibodies was done in order to evaluate cell tendency for synaptogenesis. Results: The yield of cholinergic neurons with BME as pre-inducer and NGF as inducer was 80%. Also, NF-200, NF-160, NF-68, MAP-2 and synapsin-I were detected in the transdifferentiated cells. RT-PCR showed the expression of NeuroD, while Oct-4 was not detected. Conclusion: BME as pre-inducer and NGF as inducer for BMSC transdifferentiation into cholinergic phenotype are potential sources in traumatic injury therapy in the central nervous system.
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نویسندگان مقاله مجید نقدی | majid naghdi


تقی طریحی | taki tiraihi


سید علیرضا مصباح نمین | seyed alireza mesbah namin


جلیل عرب خردمند | jalil arabkheradmand


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-26&slc_lang=en&sid=en
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