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Iranian Biomedical Journal، جلد ۱۲، شماره ۳، صفحات ۱۵۹-۱۶۵
عنوان فارسی Comparison of Distribution of Virulence Determinants in Clinical and Environmental Isolates of Vibrio cholera
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Comparison of Distribution of Virulence Determinants in Clinical and Environmental Isolates of Vibrio cholera
چکیده انگلیسی مقاله Background: The virulence of a pathogenic Vibrio cholerae is dependent on a discrete set of genetic determinants. In this study, we determined the distribution of virulence determinants among the clinical and environmental isolates of V. cholerae. Methods: The antibiotic resistance profiles of the isolates were determined using standard disk diffusion assay. PCR assay was performed to analyze the presence of toxin genes of ctx, zot and ace. The composition of cholera toxin encoding element (CTX) region flanking of the V. cholerae isolates was also analyzed. Results: All of the clinical isolates (100%) showed a complete set of virulence genes and also the attachment site of the filamentous bacteriophage CTXf. None of the environmental isolates contained the virulence genes and the attachment site of the CTXf. Analysis of the flanking regions including the toxin-linked cryptic element and repeat in toxin genes revealed their integrity in the clinical isolates while in the environmental isolates they were absent or contained incomplete sequences. Comparison of the antibiotic resistance assay of the environmental and clinical isolates showed a significant difference in the resistance profiles of the isolates obtained from the two sites. High rates of resistance to co-trimoxosol, streptomycin and chloramphenicol were found with clinical isolates. Conclusion: The absence of all virulence determinants in the environmental strains may suggest that certain ecological features must be present for V. cholerae to acquire a complete set of virulence determinants and to turn them into pathogenic strains.
کلیدواژه‌های انگلیسی مقاله Vibrio cholerae, Virulence determinants, Environment
نویسندگان مقاله بیتا بخشی | bita bakhshi


محمدرضا پورشفیع | mohammad reza pourshafie


فرح تاج نواب اکبر | farahtaj navabakbar


اکبر توکلی | akbar tavakoli


فرشته شاهچراغی | fereshteh shahcheraghi


منصور صالحی | mansoor salehi


زیبا فرج زادگان | ziba faradjzadegan


سید محسن زهرایی | seyed mohsen zahraei


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-52&slc_lang=en&sid=en
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زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده Related Fields
نوع مقاله منتشر شده مقاله کامل
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