این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Biomedical Journal، جلد ۱۱، شماره ۳، صفحات ۲۰۳-۲۰۸
عنوان فارسی جداسازی سریع DNA از مدفوع جهت تشخیص پنتیک سرطان روده
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Rapid DNA Extraction Protocol from Stool, Suitable for Molecular Genetic Diagnosis of Colon Cancer
چکیده انگلیسی مقاله Colorectal cancer (CRC) is one of the most common forms of cancers in the world and is curable if diagnosed at the early stage. Analysis of DNA extracted from stool specimens is a recent advantage to cancer diagnostics. Many protocols have been recommended for DNA extraction from stool, and almost allof them are difficult and time consuming, dealing with high amount of toxic materials like phenol. Their results vary due to sample collection method and further purification treatment. In this study, an easy and rapid method was optimized for isolating the human DNA with reduced PCR inhibitors present in stool. Methods: Fecal samples were collected from 10 colonoscopy-negative adult volunteers and 10 patients with CRC. Stool (1 g) was extracted using phenol/chloroform based protocol. The amplification of P53 exon 9 was examined to evaluate the extraction efficiency for human genomic targets and also compared its efficiency with Machiels et al. and Ito et al. protocols. Results: The amplification of exon 9 of P53 from isolated fecal DNA was possible in most cases in 35 rounds of PCR using no additional purification procedure for elimination of the remaining inhibitors. Conclusion: A useful, rapid and easy protocol for routine extraction of DNA from stool was introduced and compared with two previous protocols.
کلیدواژه‌های انگلیسی مقاله DNA Extraction, Stool, Colon Cancer
نویسندگان مقاله محمدرضا عباس زادگان | mohammad reza abbaszadegan


آرش ولایتی | arash velayati


علیرضا توسلی | alireza tavasoli


عزت دادخواه | ezzat dadkhah


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-93&slc_lang=en&sid=en
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده Related Fields
نوع مقاله منتشر شده مقاله کوتاه
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات