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Iranian Biomedical Journal، جلد ۸، شماره ۲، صفحات ۶۳-۶۸
عنوان فارسی تشکیل استر اسپرم انسان و ترتیب کروماتین اووسیت خرگوش پس از تزریق داخل سلولی اسپرم
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عنوان انگلیسی Human Sperm Aster Formation and Chromatin Configuration in Rabbit Oocytes Following Intracytoplasmic Sperm Injection Using a Piezo-Micromanipulator
چکیده انگلیسی مقاله In human fertilization, the sperm centrosome nucleates a radial array of microtubules called the sperm aster. The sperm aster is responsible for apposition of male and female pronuclei, and later gives rise to the first meiotic spindle. The objective of this study was to determine microtubule assembly and chromatin configuration in rabbit oocytes following intracytoplasmic injection with human sperm by piezo-driven pipette. Oocytes were collected from superovulated dose 14-15 h after hCG injection and were fertilized by injection of a single human sperm into the ooplasm of each oocyte without additional activation treatment. Four hours post heterologous intracytoplamic sperm injection (ICSI), rabbit eggs were fixed and microtubule organization and chromatin configuration were examined by immunofluorescence microscopy. In unfertilized oocytes, microtubules were present only in the metaphase-arrested second meiotic spindle. Following human sperm injection, an aster of microtubules formed adjacent to the sperm head, around mid-piece, and sperm aster was enlarged and assembled around male and female pronuclei. During pronuclear centration, male and female pronuclei were surrounded by a microtubule array without nucleation sites. With fertile human sperm, the sperm aster formation rate was 54.6%. From our data we concluded that human spermatozoa can be injected successfully into rabbit oocytes, resulting in a reasonable survival rate, and that rabbit oocytes provide a reliable tools for assessing human sperm centrosomal function using the Piezo-ICSI system
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نویسندگان مقاله فردین عمیدی | fardin amidi


فرید ابوالحسنی | farid abolhassani


yukihiro terada | yukihiro terada


عظیم هدایت پور | azim hedayatpour


معرفت غفاری | marefat ghaffari


هوشنگ رفیق دوست | hoshang rafigdust


sou ایچی nakamura | sou ichi nakamura


kunihiro okamura | kunihiro okamura


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-247&slc_lang=en&sid=en
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