این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Biomedical Journal، جلد ۸، شماره ۱، صفحات ۱۹-۲۳
عنوان فارسی جداسازی Herpetosiphon giganteus و آنالیز ساختمان آن به وسیله میکروسکوپ الکترونی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Isolation of Herpetosiphon giganteus and Ultrastructure Analysis by Electron Microscopy
چکیده انگلیسی مقاله Herpetosiphon giganteus is a filamentous gliding bacterium. Gliding motility is the movement of the cells over surfaces without the aid of flagella. The mechanism responsible for bacterial gliding motility has not been known and there are only a few data on Herpetosiphon giganteus. The aim of this study was to observe the ultrastructure and negative staining of isolated strains of Herpetosiphon giganteus to find any organelles of locomotion. First, 35 strains of gliding bacteria were isolated from soil, freshwater, mud and activated sewage sludge. Then, 8 strains very closely related to Herpetosiphon giganteus were used for further examination. For extracellular slime and fibril observation, photoelectron micrographs were taken from different patterns on the cell surface of strains that were negatively stained. Thin sections with and without lysozyme treatment were prepared and examined by transmission electron microscopy. When the filaments were negatively stained, fibrils were detected in young cultures. There were two different kinds of fibrils in this study. The extracellular slime of these organisms was clearly visible. Examination with the electron microscopy revealed neither flagella nor an axial filament of any kind. There was no evidence for external organelles of locomotion. The results indicated that ring like structure localized at the cell surface connected with fibrils is responsible for gliding movement. The secretion of slime is necessary for adhesion of the cell to a solid surface and for ease of movement
کلیدواژه‌های انگلیسی مقاله Gliding motility, Ultrastructure, Negative staining
نویسندگان مقاله جمیله نوروزی | jamileh nowroozi


مهدی میرزایی | mehdi mirzaii


پروانه طباطبایی | parwaneh tabatabai


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-241&slc_lang=en&sid=en
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده مقاله کامل
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات