این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Biomedical Journal، جلد ۳، شماره ۳، صفحات ۸۳-۸۶
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Construction and Expression of a Fused Gene for B Subunit of the Heat-Labile and a Truncated Form of the Heat-Stable Enterotoxins in Escherichia coli
چکیده انگلیسی مقاله Elaboration of different toxins by enterotoxigenic E. coli has been considered as one of the main virulence factors contributing to the manifestation of disease caused by these microorganisms. Various strategies have been employed to raise antibodies against these toxins as a line of defense. In this study, the 3’ terminus of the gene that codes for the binding subunit of the heat-labile enterotoxin of E. coli (LTB) was fused to the 5’ terminus of a truncated heat-stable enterotoxin of E. coli (ST) with a region coding for 7 amino acids separating the two moieties. The fused gene was sequenced and subsequently subcloned in the pET23a (+) expression vector in the EcoRI/HindIII sites. The construct was transferred into the E. coli strain, BL21 (DE3) pLysS and isopropyl-b -D-thiogalactopyranoside (IPTG) was used for induction. The expression of the LTB and ST in the fused protein was assessed using two commercially available kits. SDS-PAGE and Western blot were also used to examine the expressed protein. The results indicated the low expression of both toxin moieties that were recognizable by the commercially available antibodies and the expressed protein was non-toxic as indicated by suckling mouse assay.
کلیدواژه‌های انگلیسی مقاله Heat-labile enterotoxin, Heat-Stable enterotoxin, E. coli
نویسندگان مقاله سعید بوذری | saeid bouzari


مانا علومی | mana oloomi


علی هاتف سلمانیان | ali hatef salmaninan


انیس جعفری | anis jafati


نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-405&slc_lang=en&sid=en
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده مقاله کامل
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات