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Research Journal of Pharmacognosy، جلد ۵، شماره ۳، صفحات ۴۱-۴۶
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عنوان انگلیسی Silibinin Downregulates E-Cadherin Expression in MKN-45 Human Gastric Cancer cells
چکیده انگلیسی مقاله Background and objectives:  Gastric cancer is currently known as one of the most important causes of cancer-driven death all over the world. In patients with gastric cancer, a significant proportion of death occurs due to metastasis. On the other hand, down modulated E-cadherin level has been reported as an important contributor to tumor cell invasion and metastasis. In this regard, the present work was aimed to evaluate the impact of silibinin, a flavonolignan with established anti-tumor efficacy, on cell viability and E-cadherin expression in the gastric cancer cell line MKN-45. Methods: To determine cell viability, MTT assay was performed 48 h after silibinin treatment (at concentrations of 100, 200 and 400 μM). In addition, quantitative real-time PCR was done following total RNA extraction and cDNA synthesis, to assess E-cadherin level in cells treated with silibinin. Results: The MTT results showed concentration-dependent reducing effect of silibinin on viability of MKN-45 cells. The findings of quantitative real-time PCR analysis demonstrated upregulated E-cadherin expression in cells treated with silibinin (significantly (p≤ 0.05) at concentration of 200 μM) compared to the control cells. Conclusions: The current study suggested that silibinin may exert anti-migratory/invasive effects on gastric cancer cells by enhancing E-cadherin expression, which needs to be further investigated.  
کلیدواژه‌های انگلیسی مقاله E-cadherin, gastric cancer, MKN-45, silibinin
نویسندگان مقاله Ebrahim Faghihloo |
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

Habibollah Mirzaei |
Hepatitis Research Center, Lorestan University of Medical Sciences, Khorramabad, Iran. Department of Virology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.

Abolfazl Akbari |
Colorectal Research Center, Iran University of Medical Sciences, Tehran, Iran.

Hossein Goudarzi |
Department of Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.

نشانی اینترنتی http://www.rjpharmacognosy.ir/article_64874_5185c37374c04a6ea0ba82edb3a7af9c.pdf
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