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Research in Pharmaceutical Sciences، جلد ۱۳، شماره ۴، صفحات ۳۳۲-۳۴۲
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چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی In silico interaction of insulin-like growth factor binding protein 3 with insulin-like growth factor 1
چکیده انگلیسی مقاله Insulin-like growth factor binding protein-3 (IGFBP-3) is a vital protein exist in circulation which interacts with high affinity to insulin-like growth factor (IGFs) altering their activities. Therefore, the interaction between IGFs and IGFBP-3 has a key role altering large spectrum of activities such as cell cycle progression, proliferation and apoptosis. Despite decades of research, the crystal structure of IGFBP-3 has not been identified possibly due to some technical challenge in its crystallizing. The three-dimensional (3D) structure of IGFBP-3 was predicted using homology modeling, Phyre2, and molecular dynamic. Its interaction with IGF-1 was also identified by HADDOCK software. IGFBP-3 has the most identity with other IGFBPs in N and C-domain; however, its linker domain has lower identity. Our data predicted that IGF-1 structurally interacts with N- domain and linker domain of IGFBP-3. Some conserved residues of IGFBP-3 such as Glu33, Arg36, Gly39, Arg60, Arg66, Asn109, and Ile146 interacts with Glu3, Asp12, Phe16, Gly19, Asp20, Arg21, and Glu58 of IGF-1. In addition, our data predict that the linker domain has a loop structure which covers post translational modification and interacts with IGF-1. The phosphorylation of Ser111 in linker domain, which previously has been shown to induce apoptosis make a repulsive force interrupting this interaction to IGF-1, which enables IGFBP-3 to induce apoptosis. The present study suggests that the linker domain has a key role in recognition of IGFBP-3 with IGF-1.
کلیدواژه‌های انگلیسی مقاله Docking study,IGF-1,IGFBP-3,Linker domain,Molecular dynamic
نویسندگان مقاله | Elham Jafari
2Department of Biochemistry and Bioinformatics Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Ali Gheysarzadeh
3Biology Department, Faculty of Sciences, Shahrekord University, Shahrekord, I.R. Iran.


| Karim Mahnam
4Department of Biology, University of Payam-noor, Isfahan, I.R. Iran.


| Rezvan Shahmohammadi
2Department of Biochemistry and Bioinformatics Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Amir Ansari
2Department of Biochemistry and Bioinformatics Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Hadi Bakhtyari
2Department of Biochemistry and Bioinformatics Research Center, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.


| Mohammad Reza Mofid


نشانی اینترنتی http://rps.mui.ac.ir/index.php/jrps/article/view/1837
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/115/article-115-689403.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
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