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گوارش، جلد ۱۹، شماره ۳، صفحات ۱۷۵-۰
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عنوان انگلیسی Comparison PCR and Culture methods for detect Coccoid forms of Helicobacter pylori
چکیده انگلیسی مقاله Background: Helicobacter pylori ( Pylori ) was the most common cause of chronic gastritis and was linked to peptic ulcer disease and gastric cancer. Environmental factors such as water a reservoir of H.pylori which infect human. a Non-culture bacteria in coccoid forms widespread in aquatic environments.The objective of this study was elevating the diagnostic value of PCR and culture methods for for diagnosis coccoid forms of H.pylori . Materials and Methods: To induce coccoid forms, ten different strains of H.pylori (H 1 -H 10 ) were inoculated into 30 drinking water samples. Then, the samples were incubated at three different temperatures of 4°C, 22°C and 37°C for the durations of 30 and 60 days. The samples were cultured on brucella blood agar and DNA was also extracted also from them and PCR performed on samples Results: percentage of H.pylori cells detected at specified temperatures by the culture were 0%, 10% and 0% in the first month and were 0%,10%,30% in the second month whereas by the PCR molecular method were 30%, 80%, and 30% in the first month and were 20%, 20%, and 40% in the second month, respectively. Conclusion : finding show PCR methodis more capable than culture fordetect the coccoid forms of H.pylori , therefore this method could be used to detect non- culture forms.
کلیدواژه‌های انگلیسی مقاله Helicobacter pylori, coccoid, PCR, Culture
نویسندگان مقاله سمیه اله کرمی | somayeh allahkarami


محمد حسن شاه حسینی | mohammad hassan shahhosseiny


نسیم حیاتی رودباری | nasim hayati roodbari


داوود اسماعیلی | davood esmaili


نشانی اینترنتی http://www.govaresh.org/index.php/dd/article/view/1248
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article
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