این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
گوارش، جلد ۹، شماره ۱، صفحات ۱۱-۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Genotype Variation in H. Pylori Isolates from Iranian Patients by RAPD-PCR
چکیده انگلیسی مقاله Introduction and Aims: Do H. pylori isolates from normal and dyspeptic patients have similar genetic profiles? Since genotype variation occurs within H. pylori population with high frequency, it is tempting to exploit techniques such as RAPD-PCR to examine the possible correlation between specific H. pylori genotypes and different peptic diseases. In this study, H. pylori isolates from different dyspeptic patients were genotyped by RAPD-PCR. Materials and Methods: H. pylori isolates from 66 patients, 41 normal, 21 with ulcer, and 4 with cancer were cultured, DNAs were extracted by phenol-chloroform. RAPD-PCR was optimized, using 10-nt primers of arbitrary sequence (1281, 1254, 1247) and isolate-specific fingerprints were generated. Analysis of PCR products on agarose gel was performed using NTSYSpc program. Dendrograms were calculated according to Jaccard & Nei. Results: According to differences in genetic profiles, H. pylori isolates were clustered into 4 distinct groups: 2 groups consisted of isolates from normal patients, 2 groups of isolates from patients with ulcer, and isolates from patients with cancer were clustered along with isolates from normal a d ulcer patients. Furthermore, isolates from ulcer patients appeared in the cluster related to isolates from normal patients. Conclusions: Genetic variation is quite frequent within H. pylori populations; thus RAPD-PCR is an effective technique to reveal genetic diversity of isolates from different dyspeptic patients. In this study, H. pylori strains were clustered into 4 groups: 2 groups from normal patients, and 2 from patients with ulcer. Further studies in larger populations will help to correlate a certain peptic disease to specific strain of H. pylori.
کلیدواژه‌های انگلیسی مقاله H. pylori,Genetic diversity,RAPD-PCR,Dyspepsia diseases.
نویسندگان مقاله فریده سیاوشی | farideh siavoshi


ملیحه shokouhfard | maliheh shokouhfard


رضا ملک زاده | reza malekzadeh


نوید dinparastjadid | navid dinparastjadid


صادق massarrat | sadegh massarrat


آزاد عمرانی | azad emrani


نشانی اینترنتی http://www.govaresh.org/index.php/dd/article/view/970
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات