این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
International Journal of Radiation Research، جلد ۱۶، شماره ۲، صفحات ۱۹۷-۲۰۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Effect of dose rate on antitumor activity in hypoxic cells by using flattening filter free beams
چکیده انگلیسی مقاله Background: Recently, the Flattening Filter Free (FFF) beams, which allow a dose rate increase of up to four times compared to the normal dose rate, have been incorporated into radiation therapy machines. The aim of this study is to evaluate antitumor activity in hypoxic cells irradiated with different dose rates using FFF beams and to identify the casual mechanism for cellular damage during irradiation.  Materials and Methods: EMT6 cells were treated with 95% N2 and 5% CO2 to maintain a hypoxic condition. Three dose rates, namely, 6.27 Gy/min, 12.00 Gy/min, and 18.82 Gy/min, were used to deliver the prescribed dose of 2 to 4 Gy using the TrueBeam linear accelerator. The number of colonies was counted to evaluate the cell surviving fraction. To investigate the mode of action, additional experiments to detect reactive oxygen species (ROS) by aminophenyl fluorescein (APF) assay, and DNA double-strand breaks (DSBs) by γH2AX assay were performed. Results: Irradiation of hypoxic cells using FFF beams increases antitumor activity as a function of dose rate. The fluorescence of the APF assay was significantly increased when high dose rates were used. In addition, results from our γH2AX assays show that the number of DNA DSBs increased as a function of dose rate, in hypoxic cells. Conclusion: We demonstrate that there is a significant dose rate-dependent difference in antitumor activity in hypoxic cells, when FFF beams are used.  
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | T. Hara
Graduate School of Technology and Science, Tokushima University, Tokushima, Japan


| M. Tominaga
Institute of Health Science, Tokushima University, Tokushima, Japan


| K. Yajyu
Graduate School of Technology and Science, Tokushima University, Tokushima, Japan


| R. Kouzaki
Graduate School of Technology and Science, Tokushima University, Tokushima, Japan


| A. Hanyu
Graduate School of Technology and Science, Tokushima University, Tokushima, Japan


| H. Yamada
Institute of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan


| M. Sasaki
Institute of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan


| S. Azane
Institute of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan


| Y. Uto
Institute of Bioscience and Bioindustry, Tokushima University, Tokushima, Japan
نشانی اینترنتی http://ijrr.com/browse.php?a_code=A-10-1-717&slc_lang=en&sid=1
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/42/article-42-724315.pdf
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Radiation Biology
نوع مقاله منتشر شده تحقیق بدیع
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات