این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Medical Physics، جلد ۵، شماره Issue ۳,۴، صفحات ۱-۱۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Effect of Cell Size and Shape on Electric Field Threshold and Critical Transmembrane Voltage for Electroporation
چکیده انگلیسی مقاله Introduction: Electroporation is a technique for increasing the permeability of the cell membrane to otherwise non-permeate molecules due to an external electric field. This permeability enhancement is detectable if the induced transmembrane voltage becomes greater than a critical value which depends on the pulse strength threshold. In this study, the variability of the electric field threshold and the minimal transmembrane voltage resulting in detectable electroporation of the plasma membrane of spherical and irregularly shaped cells have been investigated. Materials and Methods: Spherical cells of different dimensions and attached cells of various shapes were selected. The chosen cells were exposed to 100 0s electric pulses with incrementing amplitudes. Electroporation was detected by an increase in the fluorescence caused by an influx of Ca 2+ and the threshold electric field for each cell was recorded. A 3D geometrical model of each cell was constructed from its cross sectional images. Simulation using the finite element method was performed to obtain the critical induced transmembrane voltage for each individual cell. Results: The magnitudes of the electric field strength threshold and critical transmembrane voltage versus cell radius were obtained for spherical cells. To investigate the effects of cell shapes and orientations on the field strength threshold and critical voltage, the considered attached cells were categorized into three different groups. Field strength threshold and critical voltage was obtained for each cells and the results for the different groups were compared. Discussion and Conclusion: Size, shape and orientation of cells affect the critical transmembrane voltage and all these elements in turn influence the electric field threshold and, therefore, the efficiency of electroporation.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله لیلا توحیدی | leila towhidi
ph.d. student, medical physics dept., tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

سید محمد pourmir فیروزآبادی | seyed mohammad pourmir firoozabadi
associate professor, medical physics dept., tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

حسین مزدارانی | hossein mozdarani
professor, medical genetics dept., tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

tadej kotnik | tadej kotnik
associate professor, faculty of electrical engineering, university of ljubljana, ljubljana, slovenia


gorazd pucihar | gorazd pucihar
post doc student, faculty of electrical engineering, university of ljubljana, ljubljana, slovenia


damijan miklavcic | damijan miklavcic
professor, faculty of electrical engineering, university of ljubljana, ljubljana, slovenia
نشانی اینترنتی http://ijmp.mums.ac.ir/article_7500.html
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Paper
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات