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Iranian Journal of Medical Sciences، جلد ۴۱، شماره ۵، صفحات ۳۸۲-۰
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عنوان انگلیسی Neural Stem Cell-based Intraocular Administration of Pigment Epithelium-derived Factor Promotes Retinal Ganglion Cell Survival and Axon Regeneration after Optic Nerve Crush Injury in Rat: An Experimental Study
چکیده انگلیسی مقاله Background: Pigment epithelium-derived factor (PEDF) is regarded as a multifunctional protein possessing neurotrophic and neuroprotective properties. PEDF has a very short half-life, and it would require multiple injections to maintain a therapeutically relevant level without a delivery system. However, multiple injections are prone to cause local damage or infection. To overcome this, we chose a cell-based system that provided sustained delivery of PEDF and compared the effect of weekly injections of PEDF and neural stem cell (NSC)-based intraocular administration of PEDF on retinal ganglion cell (RGC) survival and axon regeneration after optic nerve injury. Methods: Seventy-two rats were randomly assigned to 3 groups: group with injections of phosphate buffered saline (PBS) (n=24), group with weekly injections of PEDF (n=24), and group with NSC-based administration of PEDF (n=24). Western blot was used to analyze the PEDF protein level 2 weeks after injection. Retinal flat mounts and immunohistochemistry were employed to analyze RGC survival and axon regeneration 2 weeks and 4 weeks after injection. The data were analyzed with one-way ANOVA in SPSS (version 19.0). A P< 0.05 was considered significant. Results: The PEDF protein level in the group with NSC-based administration of PEDF increased compared with that in the groups with injections of PEDF and PBS (P< 0.05). The PEDF-modified NSCs differentiated into GFAP-positive astrocytes andβ-tubulin-III-positive neurons. NSC-based administration of PEDF effectively increased RGC survival and improved the axon regeneration of the optic nerve compared with weekly injections of PEDF. Conclusion: Subretinal space transplantation of PEDF-secreting NSCs sustained high concentrations of PEDF, differentiated into neurons and astrocytes, and significantly promoted RGC survival and axon regeneration after optic nerve injury.
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نویسندگان مقاله wei امین زانگ | wei min zhang
department of neurosurgery, zhumadian central hospital, zhumadian, china


zhi ren زانگ | zhi ren zhang
department of medical administration, zhumadian central hospital, zhumadian, china


یونگ gang زانگ | yong gang zhang
department of neurosurgery, zhumadian central hospital, zhumadian, china


یان sheng امتیاز | yan sheng gao
department of neurosurgery, zhumadian central hospital, zhumadian, china
نشانی اینترنتی http://ijms.sums.ac.ir/index.php/IJMS/article/view/2541
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده Original Article(s)
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